Image Gallery Captured by the APX100 Benchtop Fluorescence Microscope
The APEXVIEW APX100 benchtop fluorescence microscope supports a wide range of observation methods. With easy-to-use, convenient functions and intuitive software, it makes diverse research applications more efficient while not compromising on image quality.
This gallery showcases application examples captured using an APX100 digital imaging system. If you have any questions about how it can be used in your applications, let us know!
Multichannel Fluorescence
- Observe samples with multiple stains and in combination with other imaging modes like phase contrast or gradient contrast.
- Capacity of up to eight mirror cubes to accommodate many experimental conditions.
- Capture images under optimal conditions quickly with automated exposure time and Z offset calibrations for each channel.
- Neatly displays merged images to ensure each acquisition matches your standards.
BPAE cells. Stain: Mouse Anti-α-tubulin, BODIPY FL Goat Anti-Mouse IgG, Texas Red-X Phalloidin, DAPI.
Rat cerebellum labeled using 5 fluorochromes
Easily acquire multichannel fluorescence images with wavelengths ranging from UV to NIR.
Stains used: Hoechst (blue), GFAP (green), MAP2 (orange), Calbindin (red), MBP (magenta).
Objective: UPLXAPO4X
Stitching
- Capture entire tissue samples or assess the condition of cell culture flasks over wide areas rapidly at high resolution.
- High-accuracy stitching makes the joints between images nearly invisible in both brightfield and fluorescence acquisition modes.
- Even tilted or uneven samples can be cleanly stitched together.
Mouse lung captured with UPLXAPO4X objective. Stain: HE.
Z-Stack
- Acquire multiple images in the Z direction to accommodate thick samples.
- Create all-in-focus images with just a few clicks.
- Achieve sharp, blur-free images using TruSight™ 3D deconvolution.
Subcellular localization of kendrin/pericentrin, centrosome protein. Image data courtesy of Kazuhiko Matsuo, Ph.D., Division of Developmental Biology and Anatomy, Department of Anatomy, Kyoto Prefectural University of Medicine.
Time-Lapse Imaging
- Continuously record changes in a live cell or entire culture over time.
- A built-in vibration-isolation mechanism and optional incubator help ensure stable image acquisition.
- When combined with the optional drug administration unit, you can observe the response of cells immediately after drug administration in real time.
Scratch assay
Scratch assay time-lapse imaging of Human Aoritic Enodothelial Cells (HAEC) (2 days).
Image data courtesy of Dr. Kazutaka Ueda, Department of Cardiovascular Medicine, The University of Tokyo Hospital.
Fertilized mouse egg
4-hours time-lapse observation of a fertilized mouse egg.
Antioxidant effects in mitochondria
Mitochondria that are oxidized (intensity increased) by the addition of H2O2 are subsequently reduced (intensity decreased) by the antioxidant effect.
- Cell: HeLa cell
- Labeling: OxiORANGE
- Objective: UPLXAPO40X
- Time interval: 3 minutes
- Duration: 1hour
Intensity profile
Multicolor imaging of migrating RBL-2H3 cells
Three types of treated RBL-2H3 cells were mixed, and their migratory state was observed using multicolor time-lapse imaging. The cells of allt treatments showed similar migration by tracking analysis.
- Cell: RBL-2H3 cells
- Labeling: DiO(green), DiI(yellow),DiD(red)
- Objective: UPLXAPO20X
- Interval time: 30 minutes
- Duration: 18 hours
Track length: (average) μm
Fluorescence Imaging
Fluorescence in-situ hybridization (FISH) sample
Captured with a UPLXAPO60XO X Line™ objective.
Colocalization of NeuN and γ-H2AX in a monkey brain
Stain: Immunocytochemistry.
Image data courtesy of Rui Han, Lab of Professor Xiaojiang-Li, Guangdong-HongKong-Macau Institute of CNS Regeneration, Jinan University.
Hazel pollen
Autofluorescence, captured with a UPLXAPO60X oil objective. Processed with TruSight deconvolution.
Mammary gland of adult mouse (Krt14/Krt8)
Stain: Immunocytochemistry.
Image data courtesy of Chunye Liu, Lab of Prof. Yi Zeng, Center of Excellence in Molecular Cell Science, CAS.
Tubulin and nucleus of BSC-1 cells
Stain: Immunocytochemistry, White: cell nucleus, Cyan: Tubulin.
Cleared HeLa cell spheroid
Stain: Immunocytochemistry, Blue: DAPI Nucleus, Green: AF488 Ki67, Red: AF555 Actin.
Ptk2 cells
Stain: DAPI, Mitotracker Red, Acti-Stain 488.
Transverse rat brain
Stain: Hoechst, RPCA-NF-L-ct, and MCA-7D5.
Mouse kidney
Alexa Fluor 488 WGA, Alexa Fluor 568 Phalloidin, DAPI.
Brightfield Imaging
Alcian blue and nuclear fast red stained E15.5 mouse embryo
Image data courtesy of 1.2.Naoki Takeshita, MD, 1.Kenta Yashiro Professer, MD, Ph.D., 1.Division of Developmental Biology and Anatomy, Department of Anatomy and 2.Department of Pediatrics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine.
Adenoma tissue sample
Stitched brightfield image captured with a LUCPLFLN40XPH objective. Image data courtesy of the German Cancer Research Center (Deutsches Krebsforschungszentrum, DKFZ).
Xenopus blood cells
Stain: HE.
Corn stem cell
Stain: Safranin methyl blue.
Cyp26b1 expression pattern in E9.5 mouse embryo by whole mount in situ hybridization
Image data courtesy of 1.2.Naoki Takeshita, MD, 1.Kenta Yashiro Professer, MD, Ph.D., 1.Division of Developmental Biology and Anatomy, Department of Anatomy and 2.Department of Pediatrics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine.
Shepherd's purse, mature embryo capsella
Stain: Trichrome.
Gradient Contrast: See Your Sample in a New Light
- Works with any Olympus objective.
- Less affected by the meniscus, container lids, and water droplets.
- Can be used with glass- and plastic-bottom dishes and multi-well plates.
- Image through the plastic lids of Petri dishes and multi-well plates, reducing the risk of contamination.
Expression of membrane -translocated mCherry in HEK293T cell. Image data courtesy of Rie Saba, Ph.D., Division of Developmental Biology and Anatomy, Department of Anatomy, Kyoto Prefectural University of Medicine.
Images Captured with Gradient Contrast
Rat brain
HEK-293 cells
Rat testicles
