Live Super Resolution Imaging A Line Objectives and IXplore SpinSR

High-Resolution Objectives for Super Resolution

A high numerical aperture (NA) is important for super resolution images.Our proprietary polishing technology enabled us to create the world’s first plan-corrected apochromat objectives with an NA of 1.5*1. Combining this objective with the IXplore SpinSR system improves the brightness and resolution of your super resolution images. The objectives are particularly useful when visualizing surface microstructures.

*1 As of Nov. 2018, According to Olympus research.

Learn more about the IXplore SpinSR

High Resolution Objectives for Super Resolution / TIRF Application

Confocal image (left) versus super resolution image captured by UPLAPO100XOHR (right)

Scale bar: 200 nm

Super Resolution

Resolve confocal images using the confocal technique and Olympus super resolution (OSR).

Green: Alexa488 labeled Nup358 which localizes to the cytoplasmic surface of nuclear pore complex

Red: Alexa555 labeled Nup62 which localizes to the nuclear pore complex central plug

Localization of Nup358 and Nup62 can be distinguished by super resolution technique.

*Nuclear pore complex of HeLa cell
Image courtesy of: Hidetaka Kosako, Fujii Memorial Institute of Medical Sciences, Tokushima University

High-Resolution Objectives Selection Guide

Working Distance
(mm)
Magnification
Objective Field Number*2
Numerical Aperture
Immersion
Applications
UPLAPO60XOHR
0.11
60X
22
1.50
Oil
Real-time, super resolution imaging for live cells/super resolution imaging of tiny structures, such as organelles/whole cell TIRF imaging
UPLAPO100XOHR
0.12
100X
22
1.50
Oil
Real-time, super resolution, imaging for live cells/super resolution imaging of tiny structures, such as organelles/high-resolution imaging of cell membranes or subcellular organelles, and single-molecule level experiments

*2 Maximum field number observable through eyepiece

Silicone Immersion Objectives

Silicone immersion objectives are optimized for live cell and live tissue imaging. By properly matching the refractive index, images are clearer and brighter, and time−lapse observations become more reliable and less complex because silicone oil does not dry at 37 °C (98.6 °F). With a high numerical aperture and long working distance, these objectives, when combined with Olympus super resolution, enable you to observe microstructures on your sample's surface as well as deep inside it. For example, both molecule localization and nerve cell microstructures can be observed with high resolution.

Silicone immersion objectives


https://adobeassets.evidentscientific.com/content/dam/video/video/library/SpinSR10-3D-time-lapse-of-neuron_480.mp4

Image data courtesy of:Yuji Ikegaya, PhDLaboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo

3D Time-Lapse of a Neuron

Obtain detailed three-dimensional super resolution image data during time-lapse imaging.
Sample: A time-lapse image of a mouse’s primary neuron labeled by EGFP after coculture with astrocyte for 3 weeks. 0.2 µm Z-steps for 26 slices.

Silicone Immersion Objectives Selection Guide

Working Distance
(mm)
Magnification
Objective Field Number*3
Numerical Aperture
Immersion
Applications
UPLSAPO100XS
0.2
100X
22
1.35
Silicone oil
High-resolution for subcellular imaging
UPLSAPO60XS2
0.3
60X
22
1.30
Silicone oil
High-resolution and long-term, time-lapse imaging of single cells

*3 Maximum field number observable through eyepiece.

IXplore SpinSR

IXplore SpinSR

  • Super resolution down to 120 nm XY resolution
  • Prolonged cell viability in confocal time-lapse imaging due to less phototoxicity and bleaching
  • Switch between widefield, confocal, and super resolution observations in the IXplore SpinSR system in one step
  • Accurate 3D reconstruction with Olympus silicone oil immersion objectives

Learn More

*Banner Image: Fluorescent staining of microtubules (red: Alexa 594) and actin (green: Alexa 488 phalloidin) in growth cone of NG108 cells.
By courtesy of: Dr.Kaoru Katoh , Biomedical Research Institute,National Institute of Advanced Industrial Sciences and Technology