Simplifying Live-Cell Imaging

Gradient Contrast Microscopy and Smarter Observation Workflows

Transparent specimens can be difficult to observe during live-cell imaging, particularly when conventional phase visualization methods introduce limitations involving thick samples and plastic vessels.

In this ebook, we examine how gradient contrast microscopy addresses these challenges using an optimized optical approach to support clearer observation of unstained specimens.

What You'll Learn in This Ebook

• An overview of common phase visualization methods
• How gradient contrast microscopy works
• Imaging considerations for thick specimens and plastic vessels
• Workflow limitations associated with conventional observation methods
• How the APX100 microscope supports faster setup using macro imaging, automated positioning, and AI-assisted sample recognition

Pre-observation setup can influence the pace of the entire imaging workflow.