Mirror Units

Fluorescence Filter Cubes

Contact Us

Overview

Olympus UIS2 fluorescence mirror units are optimized to maximize fluorophore performance, producing uniform and highly efficient light transmittance. The filter efficiency enables the use of weaker excitation light to minimize photobleaching and sample damage.

Filter Cubes for BX3, IX3 Microscopes, and APX100 system

Download the transmittance wavelength data for U-F Series

Unit Name
Excitation Filter
Emission Filter
Dichromatic Mirror
Ultraviolet Excitations
U-FUW
340-390
420IF
410
U-FUN
360-370
420IF
410
U-FUNA
360-370
420-460
410
Blue Violet Excitations
U-FBVW
400-440
460IF
455
Blue Excitations
U-FBW
460-495
510IF
505
U-FBWA
460-495
510-550
505
U-FBN
470-495
510IF
505
U-FBNA
470-495
510-550
505
Green Excitations
U-FGW
530-550
575IF
570
U-FGWA
530-550
575-625
570
U-FGNA
540-550
575-625
570
Yellow Excitations
U-FYW
540-585
600IF
595
Fluorescent Protein Variants
U-FCFP
425-445CFP
460-510CFP
455CFP
U-FGFP
460-480GFP
495-540GFP
490GFP
U-FYFP
490-500YFP
515-560YFP
515YFP
U-FRFP
535-555HQ
570-625HQ
565HQ
U-FMCHE
565-585
600-690
595

Filter Cubes for IX3 Microscopes

Unit Name
Excitation Filter
Emission Filter
Dichromatic Mirror
Ultraviolet Excitations
IX3-FUWXL
340-390
420IF
410
Blue Violet Excitations
IX3-FBVWXL
400-440
460IF
455
Green Excitations
IX3-FGWXL
530-550
575IF
570
Fluorescent Protein Variants
IX3-FCFPXL
425-445CFP
460-510CFP
455CFP
IX3-FGFPXL
460-480GFP
495-540GFP
490GFP
IX3-FYFPXL
490-500YFP
515-560YFP
515YFP
IX3-FMCHEXL
565-585
600-690
595

Filter Cubes for IX2, BX2, and MVX10 Microscopes

Download the transmittance wavelength data for U-M Series

Unit Name
Excitation Filter
Emission Filter
Dichromatic Mirror
Ultraviolet Excitations
U-MWU2
330-385
420
400
U-MNU2
360-370
420
400
U-MNUA2
360-370
420-460
400
Violet Excitations
U-MNV2
400-410
455
455
Blue Violet Excitations
U-MWBV2
400-440
475
455
U-MNBV2
420-440
475
455
Blue Excitations
U-MSWB2
420-480
520IF
500
U-MWB2
460-490
520IF
500
U-MNB2
470-490
520IF
500
U-MWIB3
460-495
510IF
505
U-MWIBA3
460-495
510-550
505
U-MNIB3
470-495
510IF
505
U-MNIBA3
470-495
510-550
505
Green Excitations
U-MWG2
510-550
590
570
U-MNG2
530-550
590
570
U-MWIG3
530-550
575IF
570
U-MWIGA3
530-550
575-625
570
U-MNIGA3
540-550
575-625
570
Yellow Excitations
U-MWIY2
545-580
610IF
600
Fluorescent Protein Variants
U-MCFPHQ
425-445HQ
460-510HQ
450
U-MGFPHQ
460-480HQ
495-540HQ
485
U-MYFPHQ
490-500HQ
515-560HQ
505
U-MRFPHQ
535-555HQ
570-625HQ
565

Selection Chart

Fluorescent Protein Variants

Excitation Maximum (nm)
Emission Maximum (nm)
Applicable BX3/IX3/APX100 Mirror Unit
Applicable BX2WI/MVX10 Mirror Unit
AmCyan
458
489
U-FCFP, U-FBVW
U-MWBV2, U-MNBV2, U-MCFPHQ
Azami-Green
492
505
U-FBWA, U-FBNA, U-FGFP, U-FBW, U-FBN
U-MWIB3, U-MWIBA3, U-MNIB3, U-MNIBA3, U-MGFPHQ
DsRed2
563
582
U-FGW, U-FGWA, U-FGNA, U-FRFP
U-MWIG3, U-MWIGA3, U-MNIGA3, U-MRFPHQ
EBFP
380
440
U-FUNA, U-FUW, U-FUN
U-MWU2, U-MNU2, U-MNUA2, U-MNV2
ECFP
433
475
U-FCFP, U-FBVW
U-MCFPHQ, U-MWBV2, U-MNBV2, U-MNV2
EGFP
488
507
U-FBWA, U-FBNA, U-FGFP, U-FBW, U-FBN
U-MGFPHQ, U-MNIB3, U-MWIBA3, U-MWIB3, U-MNIBA3
EYFP
513
527
U-FBWA, U-FBNA, U-FYFP, U-FBW, U-FBN
U-MYFPHQ, U-MWIBA3, U-MNIBA3, U-MWIBA3, U-MNIBA3, U-MNIB3, U-MNB2, U-MWB2, U-MWIB3
Fucci (mAG1)
492/551
505/565
U-FBNA, U-FGFP, U-FBWA, U-FBW, U-FBN
U-MNIBA3, U-MGFPHQ, U-MNIB3, U-MWIBA3, U-MWIB3
GFP (S65T)
488
510
U-FBWA, U-FBNA, U-FGFP, U-FBW, U-FBN
U-MGFPHQ, U-MWIBA3, U-MNIBA3, U-MWIB3, U-MNIB3
GFP (WT)
395, 475
510
U-FBWA, U-FBNA, U-FGFP, U-FBW, U-FBN
U-MWIBA3, U-MNIBA3, U-MGFPHQ, U-MWIB3, U-MNIB
HcRed
588
618
U-FYW
U-MWIY2
Midoriishi-Cyan
472
495
U-FBWA, U-FBW, U-FBNA, U-FBN, U-FCFP, U-FGFP
U-MWIBA3, U-MWIB3, U-MNIBA3, U-MNIB3, U-MCFPHQ, U-MGFPHQ
mCherry
580
610
U-FMCHE, U-FYW
U-MWIY2
mRFP
584
607
U-FMCHE, U-FYW
U-MWIY2
mStrawberry
574
596
U-FMCHE, U-FYW
U-MWIY2
RFP (DsRed)
558
583
U-FRFP, U-FGW, U-FGWA, U-FGNA
U-MRFPHQ, U-MWIG3, U-MWIGA3, U-MNIGA3
Venus
515
528
U-FYFP
U-MYFPHQ, U-MNB2, U-MNIB3, U-MNIBA3, U-MSWB2, U-MWB2, U-MWIB3, U-MWIBA3

Photoactivation

Excitation Maximum (nm)
Emission Maximum (nm)
Applicable BX3/IX3/APX100 Mirror Unit
Applicable BX2WI/MVX10 Mirror Unit
Dronpa-Green
503
518
U-FBN, U-FBNA, U-FBW, U-FBWA, U-FYFP
U-MGFPHQ, U-MNB2, U-MNIB3, U-MNIBA3, U-MWB2, U-MWIB3, U-MWIBA3, U-MYFPHQ
PA-GFP
504
517
U-FGFP, U-FBWA, U-FBNA, U-FBW, U-FBN
U-MGFPHQ, U-MNIB3, U-MWIBA3, U-MWIB3, U-MNIBA3

Photoconversion

Excitation Maximum (nm)
Emission Maximum (nm)
Applicable BX3/IX3/APX100 Mirror Unit
Applicable BX2WI/MVX10 Mirror Unit
Kaede (Green/Red)
509/573
518/582
U-FBN, U-FBNA, U-FBW, U-FBWA, U-FGFP,
U-FYFP, U-FGW, U-FGWA, U-FGNA, U-FRFP
U-MWIB3, U-MWIBA3, U-MGFPHQ, U-MYFPHQ, U-MNB2, U-MNIB3, U-MNIBA3,U-MWB2, U-MNIGA3, U-MRFPHQ, U-MWIG3, U-MWIGA3

Carbohydrate Modification, Protein Modification (Labeling)

Protein modification

Excitation Maximum (nm)
Emission Maximum (nm)
Applicable BX3/IX3/APX100 Mirror Unit
Applicable BX2WI/MVX10 Mirror Unit
Alexa Fluor 350
346
442
U-FUNA, U-FUW, U-FUN
U-MNUA2, U-MWU2, U-MNU2
Alexa Fluor 405
402
421
U-FUNA, U-FUW, U-FUN
U-MNUA2, U-MWU2, U-MNU2
Alexa Fluor 430
434
539
U-FBVW
U-MWBV2, U-MNBV2, U-MNV2
Alexa Fluor 488
495
519
U-FBWA, U-FBNA, U-FBW, U-FBN
U-MWIBA3, U-MNIBA3, U-MWIB3, U-MNIB3,UMNB2, U-MWB2
Alexa Fluor 532
531
554
U-FGW, U-FGWA, U-FGNA, U-FRFP
U-MWIG3, U-MWIGA3, U-MNIGA3, U-MRFPHQ
Alexa Fluor 546
556
573
U-FGW, U-FGWA, U-FGNA, U-FRFP
U-MWIG3, U-MWIGA3, U-MNIGA3, U-MRFPHQ
Alexa Fluor 555
555
567
U-FGW, U-FGWA, U-FGNA, U-FRFP
U-MWIG3, U-MWIGA3, U-MNIGA3, U-MRFPHQ
Alexa Fluor 568
578
603
U-FGW, U-FGWA, U-FGNA, U-FRFP, U-FYW, U-MCHE
U-MWIG3, U-MWIGA3, U-MNIGA3, U-MRFPHQ, U-MWIY2
Alexa Fluor 594
590
617
U-FYW, U-FMCHE
U-MWIY2
Allophycocyanin (APC)
650
660
U-FYW
U-MWIY2
7-amino-4-methylcoumarin-3-acetic acid (AMCA)
349
448
U-FUNA, U-FUW, U-FUN
U-MNUA2, U-MWU2, U-MNU2
BODIPY FL
505
513
U-FBWA, U-FBW, U-FBNA, U-FBN, U-FGFP
U-MWIBA3, U-MWIB3, U-MNIBA3, U-MNIB3
Cascade Blue
376
425
U-FUNA, U-FUW, U-FUN
U-MNUA2, U-MWU2, U-MNU2
Fluorescein-isothiocyanate (FITC)
494
518
U-FBWA, U-FBW, U-FBNA, U-FBN, U-FGFP, U-FYFP
U-MWIBA3, U-MNIBA3, U-MWB2, U-MNB2, U-MWIB3, U-MNIB3, U-MGFPHQ, U-MYFPHQ
Oregon Green 488
496
524
U-FBWA, U-FBW, U-FBNA, U-FBN, U-FGFP, U-FYFP
U-MWIBA3, U-MNIBA3, U-MWB2, U-MNB2, U-MWIB3, U-MNIB3, U-MGFPHQ, U-MYFPHQ
Phycoerythrin B (PE-B)
545
576
U-FGW, U-FGWA, U-FGNA, U-FRFP
U-MWIG3, U-MWIGA3, U-MNIGA3, U-MRFPHQ
Phycoerythrin R (PE-R)
490, 565
578
U-FGNA, U-FGW, U-FGWA, U-FRFP
U-MNIGA3, U-MWIG3, U-MWIGA3, U-MRFPHQ
Rhodamine B-isothiocyanate (RITC)
543
580
U-FGW, U-FGWA, U-FGNA, U-FRFP
U-MWIG3, U-MWIGA3, U-MNIGA3, U-MRFPHQ
Texas Red
595
615
U-FYW, U-MCHE
U-MWIY2
Tetramethylrhodamine-isothyocyanate (TRITC)
541
572
U-FGW, U-FGWA, U-FGNA, U-FRFP
U-MWIG3, U-MWIGA3, U-MNIGA3, U-MRFPHQ
1-Anilinonaphthalene-8-sulfonic acid (ANS)
365
460
U-FUW, U-FUN
U-MWU2, U-MNU2
CPM (7-Diethylamino-3-(4'-Maleimidylphenyl)-4-Methylcoumarin)
~384
470
U-FUW, U-FUN
U-MWU2, U-MNU2
Cy2
489
506
U-FBWA, U-FBNA, U-FBW, U-FBN
U-MWIBA3, U-MNIBA3, U-MWIB3, U-MNIB3
Cy3
(512); 550
570; (615)
U-FGW, U-FGWA, U-FGNA
U-MWIG3, U-MWIGA3, U-MNIGA3
Cy3.5
581
594; (640)
U-FYW, U-FMCHE
U-MWIY2
Dans　(1-Dimethyl Amino Naphaline 5 Sulphonic Acid)
340
525
U-FUW, U-FUN
U-MWU2, U-MNU2
Dansyl chloraide
360
520
U-FUW, U-FUN
U-MWU2, U-MNU2
Dansyl hydrazine
340
525
U-FUW, U-FUN
U-MWU2, U-MNU2
Eosin
525
545
U-FBWA, U-FBNA, U-FBW, U-FBN
U-MWIBA3, U-MNIBA3, U-MWIB3, U-MNIB3
Fluorescamine
390
465/475
U-FUW, U-FUN, U-FNUA
U-MWU2, U-MNU2, U-MNUA2, U-MNV2
FluorX
494
520
U-FBWA, U-FBNA, U-FBW, U-FBN
U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3, U-MSWB2, U-MWB2, U-MNB2
Lucifer Yellow CH
425
528
U-FBVW, U-FBW, U-FBW, U-FBWA
U-MWBV2, U-MNBV2, U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3, U-MWB2, U-MNIB2, U-MSWB2
Monochlorobimane (mBCI)
394
490
U-FUW, U-FUN, U-FUNA
U-MNUA2, U-MWU2, U-MNU2, U-MNV2
o-phtalaldehyde (OPT)
340
455
U-FUNA, U-FUW, U-FUN
U-MNUA2, U-MWU2, U-MNU2
Resorufin
572
583
U-FGW, U-FGWA, U-FGNA
U-MWIG3, U-MWIGA3, U-MNIGA3, U-MWG2, U-MNG2
SITS (Primuline)
395-425
450
U-FUW, U-FUN, U-FUNA
U-MNUA2, U-MWU2, U-MNU2, U-MNV2
SITS (Stilbene Isothiosulphonic acid)
365
460
U-FUW, U-FUN, U-FUNA
U-MNUA2, U-MWU2, U-MNU2

Carbohydrate modification

Excitation Maximum (nm)
Emission Maximum (nm)
Applicable BX3/IX3/APX100 Mirror Unit
Applicable BX2WI/MVX10 Mirror Unit
Acrifl avine
436
520
U-FBVW, U-FBW
U-MWBV2, U-MNBV2, U-MWB2, U-MSWB2

Nuclei, Nucleic Acid Staining

Nuclei, nucleic acid

Excitation Maximum (nm)
Emission Maximum (nm)
Applicable BX3/IX3/APX100 Mirror Unit
Applicable BX2WI/MVX10 Mirror Unit
Acridine Orange
500
530/630-650
U-FBW, U-FBN, U-FBWA, U-FBNA, U-FYFP
U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3
Acridine Yellow
470
550
U-FBW, U-FBN, U-FBWA, U-FBNA
U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3, U-MWB2, U-MNIB2, U-MSWB2
Acrifl avine-Feulgen
455
515
U-FBVW, U-FBW, U-FBWA
U-MWBV2, U-MNBV2, U-MWIB3, U-MWIBA3, U-MWB2, U-MSWB2
Auramine O-Feulgen
460
550
U-FBW, U-FBWA, U-FBNA
U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3, U-MWB2, U-MNIB2, U-MSWB2
7-Amino Actinomycin D
546
647
U-FYW, U-FGW, U-FMCHE
U-MWIG3, U-MWG2, U-MNG2, U-MWIY2
bis-Aminophenyl-oxadizole (BAO) -Feulgen
380
470
U-FUW, U-FUN, U-FUNA
U-MWU2, U-MNU2, U-MNUA2
BOBO 1
462
481
U-FBVW
U-MWBV2, U-MNBV2, U-MCFPHQ
BO PRO 1
462
481
U-FBVW
U-MWBV2, U-MNBV2
Chromomycin A3
445
575
U-FBVW
U-MWBV2, U-MNBV2, U-MSWB2
4, 6-diamidino-2-phenyl-indole HCI (DAPI)
345
455
U-FUW, U-FUN, U-FUNA
U-MWU2, U-MNU2, U-MNUA2
Ethidium Bromide
493
620
U-FGW, U-FGWA, U-FGNA
U-MWIG3, U-MWIGA3, U-MNIGA3, U-MWG2, U-MNG2, U-SWG2
Hoechst 33258
345
478
U-FUW, U-FUN, U-FUNA
U-MWU2, U-MNU2, U-MNUA2
Hoechst 33342
343
483
U-FUW, U-FUN, U-FUNA
U-MWU2, U-MNU2, U-MNUA2
Mithramycin
445
575
U-FBVW
U-MWBV2, U-MNBV2, U-MSWB2
Olivomycin
430
545
U-FBVW
U-MWBV2, U-MNBV2
Pararosaniline-Feulgen
570
625
U-FYW, U-FMCHE, U-FGW, U-FGWA, U-FGNA
U-MWIY2, U-MWG2, U-MNG2, U-MWIG3, U-MWIGA3, U-MNIGA3
POPO 1
434
456
U-FBVW, U-FCFP
U-MNV2, U-MWBV2, U-MNBV2, U-MCFPHQ
Propidium Iodide (PI)
536
617
U-FGW, U-FGWA, U-FGNA, U-FYW
U-MWIG3, U-MWIGA3, U-MNIGA3, U-MWIY2
Pyronin Y
540
570
U-FGW, U-FGWA, U-FGNA
U-MWIG3, U-MWIGA3, U-MNIGA3
Quinacrine Mustard
423
503
U-FBVW
U-MWBV2, U-MNBV2
SYTOX Green nucleic acid stain
504
523
U-FBW, U-FBN, U-FBWA, U-FBNA
U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3
Thiazole Orange
510
530
U-FBW, U-FBN, U-FBWA, U-FBNA
U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3
TOTO 1
509
533
U-FBW, U-FBN, U-FBWA, U-FBNA
U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3
TOTO 3
642
661
U-FYW, U-FMCHE
U-MWIY2
TO PRO 3
642
661
U-FYW, U-FMCHE
U-MWIY2
YO PRO 1
491
509
U-FBW, U-FBN, U-FBWA, U-FBNA
U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3
YOYO 1
491
509
U-FBW, U-FBN, U-FBWA, U-FBNA, U-FGFP, U-FYFP
U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3

Cell Viability Assessment

Living cell staining

Excitation Maximum (nm)
Emission Maximum (nm)
Applicable BX3/IX3/APX100 Mirror Unit
Applicable BX2WI/MVX10 Mirror Unit
Acridine Orange
500
530/630-650
U-FBW, U-FBN, U-FBWA, U-FBNA, U-FYFP
U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3
BCECF indicator
482(low pH)
503(high pH)
520(low pH)
528 (high pH)
U-FBW, U-FBN, U-FBWA, U-FBNA
U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3
Calcein
494
517
U-FBW, U-FBN, U-FBWA, U-FBNA
U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3
Carboxyfl uorescein diacetate (CFDA)
496
515
U-FBW, U-FBN, U-FBWA, U-FBNA
U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3
Fluorescein diacetate (FDA)
496
515
U-FBW, U-FBN, U-FBWA, U-FBNA
U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3

Dead cell staining

Excitation Maximum (nm)
Emission Maximum (nm)
Applicable BX3/IX3/APX100 Mirror Unit
Applicable BX2WI/MVX10 Mirror Unit
Ethidium Bromide
493
620
U-FGW, U-FGWA, U-FGNA
U-MWIG3, U-MWIGA3, U-MNIGA3, U-MWG2, U-MNG2, U-SWG2
Ethidium homodimer-1
528
617
U-FGW, U-FGWA, U-FGNA, U-FYW
U-MWG2, U-MNG2, U-MWIG3, U-MWIGA3, U-MNIGA3
Propidium Iodide (PI)
536
617
U-FGW, U-FGWA, U-FGNA, U-FYW
U-MWIG3, U-MWIGA3, U-MNIGA3, U-MWIY2

Membranes, Organelle, Membrane Potential

Membranes

Excitation Maximum (nm)
Emission Maximum (nm)
Applicable BX3/IX3/APX100 Mirror Unit
Applicable BX2WI/MVX10 Mirror Unit
8-Anilino-1-naphthalene sulfonic acid (1,8-ANS)
385
485
U-FUW, U-FUN, U-FUNA
U-MWU2, U-MNU2, U-MNUA2
Dil
550
565
U-FGW, U-FGWA, U-FGNA
U-MWIG3, U-MWIGA3, U-MNIGA3
DiO
484
501
U-FBW, U-FBN, U-FBWA, U-FBNA
U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3, U-MSWB, U-MWB2, U-MNB2
Diphenylhexatriene (DPH)
351
430
U-FUW, U-FUN, U-FUNA
U-MWU2, U-MNU2, U-MNUA2
Filipin
350
480
U-FUW, U-FUN, U-FUNA
U-MWU2, U-MNU2, U-MNUA2
Fluorescamine
390
465
U-FUW, U-FUN
U-MWU2, U-MNU2, U-MNV2
Merocyanine 540
500
580
U-FGW
U-MWG2, U-MNG2, U-MWIG3

Organelle

Excitation Maximum (nm)
Emission Maximum (nm)
Applicable BX3/IX3/APX100 Mirror Unit
Applicable BX2WI/MVX10 Mirror Unit
Acridine Orange
500
530/630-650
U-FBW, U-FBN, U-FBWA, U-FBNA, U-FYFP
U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3
BODIPY FL Ceramide
503
512
U-FBW, U-FBN, U-FBWA, U-FBNA
U-MWIB3,U-MNIB3, U-MWIBA3, U-MNIBA3
DiO C6(3)
484
500
U-FBW, U-FBN, U-FBWA, U-FBNA
U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3, U-MSWB, U-MWB2, U-MNB2
ER Tracker Blue-White DPX
374
575
U-FUW, U-FUN
U-MWU2, U-MNU2, U-MNV2
JC-1
485
530
U-FBW, U-FBN, U-FBWA, U-FBNA
U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3, U-MSWB, U-MWB2, U-MNB2
Lyso Tracker Red DND-99
577
590
U-FGW, U-FGWA, U-FGNA, U-FYW
U-MWIG3, U-MWIGA3, U-MNIGA3, U-MWIY2
Mito Tracker Green
490
516
U-FBW, U-FBN, U-FBWA, U-FBNA
U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3
Mito Tracker Red CMXRos
579
599
U-FGW, U-FGWA, U-FGNA, U-FYW
U-MWIG3, U-MWIGA3, U-MNIGA3, U-MWIY2
Nile Red (phospholipid)
552
636
U-FGW, U-FGWA, U-FGNA, U-FYW
U-MWG2, U-MNG2, U-MWIG3, U-MWIGA3, U-MNIGA3, U-MWIY2
Rhodamine 123
507
530
U-FBW, U-FBN, U-FBWA, U-FBNA
U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3
Pyronin Y
540
570
U-FGW, U-FGWA, U-FGNA
U-MWIG3, U-MWIGA3, U-MNIGA3, U-MWG2, U-MNG2

Membrane potential

Excitation Maximum (nm)
Emission Maximum (nm)
Applicable BX3/IX3/APX100 Mirror Unit
Applicable BX2WI/MVX10 Mirror Unit
Di-4-ANEPPS
497
710
U-FBW, U-FBN
U-MWIB3, U-MNIB3
Di-8-ANEPPS
465
635
U-FBW, U-FBN
U-MWIB3, U-MNIB3
FM 1-43
473
579
U-FBW, U-FBN
U-MWIB3, U-MNIB3
RH160
577
>610
U-FGW, U-FGWA, U-FGNA, U-FYW
U-MWIG3, U-MWIGA3, U-MNIGA3, U-MWIY2
RH414
490
>662
U-FBW, U-FBN
U-MWIB3, U-MNIB3
RH421
541
>590
U-FGW, U-FGWA, U-FGNA
U-MWIG3, U-MWIGA3, U-MNIGA3
RH423
520-550
>610
U-FGW, U-FGWA, U-FGNA
U-MWIG3, U-MWIGA3, U-MNIGA3
RH795
560
650
U-FGW, U-FYW
U-MWIG3, U-MWIY2

Bacteria Staining, Bone Staining

Bacteria

Excitation Maximum (nm)
Emission Maximum (nm)
Applicable BX3/IX3/APX100 Mirror Unit
Applicable BX2WI/MVX10 Mirror Unit
Acridine Orange
500
530/630-650
U-FBW, U-FBN, U-FBWA, U-FBNA, U-FYFP
U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3
Acridine Yellow
470
550
U-FBW, U-FBN, U-FBWA, U-FBNA
U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3, U-MWB2, U-MNIB2, U-MSWB2
Auramine O
460
550
U-FBVW, U-FBW
U-MWBV2, U-MNBV2, U-MSWB2, U-MWB2, U-MWIB, U-MWIB3
Berberine sulfate
430
550
U-FBVW
U-MWBV2, U-MNBV2
Coriphosphine O
460
575
U-FBW
U-MWB2, U-MWIB3
CTC
430, 480
630
U-FBVW, U-FBW, U-FBN
U-MWBV2, U-MNBV2, U-MWIB3, U-MNIB3
Hoechst 33258
365
465
U-FUW, U-FUN, U-FUNA
U-MWU2, U-MNU2, U-MNUA2

Bone

Excitation Maximum (nm)
Emission Maximum (nm)
Applicable BX3/IX3/APX100 Mirror Unit
Applicable BX2WI/MVX10 Mirror Unit
Acidic Fuchsin (Pararosaniline)
540
630
U-FGW, U-FGN
U-MWG2, U-MNG2, U-MWIG3
Calcein
495
520
U-FBW, U-FBN, U-FBWA, U-FBNA
U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3
Calcein Blue
375
435
U-FUW, U-FUN, U-FUNA
U-MWU2, U-MNU2, U-MNUA2
Tetracycline
390
560
U-FUW, U-FBVW
U-MWU2, U-MNV2, U-MWBV2, U-MNBV2
Xylenol Orange
440, 570
610
U-FGW, U-FGN
U-MWG2, U-MNG2, U-MWIG3

Ion Concentrations

Calcium

Excitation Maximum (nm)
Emission Maximum (nm)
Applicable BX3/IX3/APX100 Mirror Unit
Applicable BX2WI/MVX10 Mirror Unit
BTC
400/480
540
U-FBN, U-FBNA, U-FBW, U-FBWA
U-MNV, U-MNIB3, U-MNIBA3, U-MWIB3, U-MWIBA3
Calcium Green
505
532
U-FBN, U-FBNA, U-FBW, U-FBWA
U-MNIB3, U-MNIBA3, U-MWIB3, U-MWIBA3
Fluo 4
495
510
U-FBN, U-FBNA, U-FBW, U-FBWA
U-MNIB3, U-MNIBA3, U-MWIB3, U-MWIBA3
Oregon Green 488 BAPTA-1
494
523
U-FBN, U-FBNA, U-FBW, U-FBWA
U-MNIB3, U-MNIBA3, U-MWIB3, U-MWIBA3
Oregon Green 488 BAPTA-5N
494
521
U-FBN, U-FBNA, U-FBW, U-FBWA
U-MNIB3, U-MNIBA3, U-MWIB3, U-MWIBA3

pH

Excitation Maximum (nm)
Emission Maximum (nm)
Applicable BX3/IX3/APX100 Mirror Unit
Applicable BX2WI/MVX10 Mirror Unit
BCECF
440/490
530
U-FBVW, U-FBN, U-FBNA, U-FBW, U-FBWA
U-MWBV, U-MNIB3, U-MNIBA3, U-MWIB3, U-MWIBA3

Nerve Cell Tracer, Others

Nerve cell tracer

Excitation Maximum (nm)
Emission Maximum (nm)
Applicable BX3/IX3/APX100 Mirror Unit
Applicable BX2WI/MVX10 Mirror Unit
Evans Blue
470-540
680
U-FBW, U-FBN
U-MWIB3, U-MNIB3
Fast Blue
360
410
U-FUW, U-FUN, U-FUNA
U-MWU2, U-MNU2, U-MNUA2
Granular Blue
375
410
U-FUW, U-FUN, U-FUNA
U-MWU2, U-MNU2, U-MNUA2
True Blue
373
404
U-FUW, U-FUN, U-FUNA
U-MWU2, U-MNU2, U-MNUA2

Others

Excitation Maximum (nm)
Emission Maximum (nm)
Applicable BX3/IX3/APX100 Mirror Unit
Applicable BX2WI/MVX10 Mirror Unit
Aniline blue
370
509
U-FUW, U-FUN
U-MWU2, U-MNU2
Alexa Fluor 488-Phalloidin
495
518
U-FBW, U-FBN, U-FBWA, U-FBNA
U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3
Calcofl uor white
440
500-520
U-FUW, U-FUN
U-MWU2, U-MNU2, U-MNV2
Euchrysine (Acridine Orange)
500
530/630-650
U-FBW, U-FBN, U-FBWA, U-FBNA, U-FYFP
U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3
NanoOrange
485
590
U-FBW, U-FBN
U-MWIB3, U-MNIB3
NBD-Phallacidin
465
536
U-FBW, U-FBN, U-FBWA, U-FBNA
U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3
Rhodamine-Phalloidin
540
565
U-FGW, U-FGWA, U-FGNA
U-MWIG3, U-MWIGA3, U-MNIGA3, U-MWG2, U-MNG2
sulforhodamine 101
586
605
U-FYW, U-FMCHE
U-MWIY2
Thiofl avin T
430
485
U-FBVW
U-MWBV2, U-MNBV2
Mirror Units