FoLu Cells Labeled for F-Actin with an Alexa Fluor Probe Conjugated to a Phallotoxin

The culture of FoLu fibroblasts presented in this section was labeled for mitochondria with MitoTracker Red CMXRos and for the cytoskeletal filamentous actin network with Alexa Fluor 488 conjugated to phalloidin, a cyclic peptide derived from the toxicdeath capmushroom (Amanita phalloides). In addition, the cells were probed for DNA in the cell nucleus with TO-PRO-3, a monomeric cyanine nucleic acid stain.