Brief Overview of Fluorescence
The phenomenon of fluorescence was known by the middle of the nineteenth century. British scientist Sir George G. Stokes first made the observation that the mineral fluorspar exhibits fluorescence when illuminated with ultraviolet light, and he coined the word "fluorescence". Stokes observed that the fluorescing light has longer wavelengths than the excitation light, a phenomenon that has become to be known as the Stokes shift. Fluorescence microscopy is an excellent method of studying material that can be made to fluoresce, either in its natural form (termed primary or autofluorescence) or when treated with chemicals capable of fluorescing (known as secondary fluorescence). The fluorescence microscope was devised in the early part of the twentieth century by August Köhler, Carl Reichert, and Heinrich Lehmann, among others. However, the potential of this instrument was not realized for several decades, and fluorescence microscopy is now an important (and perhaps indispensable) tool in cellular biology.
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Fluorescence Reference Listing
The reference materials listed below were utilized in the construction of the introductory fluorescence section in the Molecular Expressions Microscopy Primer.
Brief Overview of Fluorescence
Introduction to Fluorescence
Fluorescence microscopy is a rapidly expanding and invaluable tool of investigation. Its advantages are based upon attributes not as readily available in other optical microscopy techniques.
Overview of Excitation and Emission Fundamentals
To achieve maximum fluorescence intensity, the fluorochrome is excited at the wavelength at the peak of the excitation curve, and the emission is selected at the peak wavelength of the emission curve.