Deconvolution Resources
Web Articles
3-D Doctor
3D-Doctor, written and distributed by Able Software Corporation, is an advanced 3D image visualization, rendering and measurement software package designed for microscopy and related imaging applications. The software creates 3D surface model and volume rendering from two-dimensional cross-section images using PC platforms.
Applied Precision
Designers of theDeltaVisionRestoration Microscopy System, offers a complete hardware and software package to assist the microscopist in obtaining high-quality digital images from fluorescent specimens. The package consists of deconvolution software, microscope light path and stage control hardware, and custom optical filters.
AutoQuant Imaging
AutoQuant offers three powerful software pages designed for advanced image processing using deconvolution and three-dimensional optical microscopy techniques.AutoDebluris a deconvolution software package developed for the Windows platform that removes haze, blur, noise, and other artifacts from two- and three-dimensional micrographs. Among AutoQuant's three-dimensional software programs is the powerfulAutoVisualize-3D, which is a rendering and visualization program suited for inspecting biomedical imagery, such as optically sectioned widefield micrographs. A second program,OcuView-3Dis designed to compensate for motion (shift and rotation) of specimens that are alive and moving.
Intelligent Imaging Innovations
The company, more commonly known as3i, designs and produces high-end, user-friendly digital imaging systems and software that integrate state-of-the-art automated fluorescence microscopes with powerful multi-dimensional data acquisition and analysis.
W. M. Keck Center for Cellular Imaging
Scientists at the Keck Center have built an educational website dealing with a variety of aspects in optical microscopy. Included are tutorials on deconvolution, confocal microscopy, and multiphoton microscopy. The tutorials are accompanied by an image library and lists of selected references.
Scanalytics Deconvolution
Scanalytics, Inc., a subsidiary corporation of CSP, Inc., offers several software packages for deconvolution microscopy. The flagship program, Exhaustive Photon Reassignment, is a fully integrated system for image acquisition and analysis which provides quantitatively accurate, high resolution 2D and 3D images of fluorescently probed microscopic specimens. Other packages for both the Windows and Macintosh platforms supply algorithms for deconvolution, volume rendering, and deblurring.
Scientific Volume Imaging
Specializing in deconvolution and volume rendering software, this company offers several software packages available to the microscopist. TheHuygens Professionalis a deconvolution application that enables restoration of widefield and confocal 2D, 3D and 4D (time series) images using a UNIX platform that operates on Silicon Graphics computers or LINUX on PC's. TheHuygens Essentialis based on the same compute engine but has a wizard driven user interface that makes deconvolution very easy. It is available for WINDOWS, LINUX and IRIX.
Scientific Volume Imaging: FreeSFP
Based on a physically realistic light propagation model, the SFP renderer allows you to view your raw and deconvolved multi-channel 3D datasets in a very intuitive manner. This freeware is downloadable from SVI's website.
John W. Sedat Laboratory
Professor Sedat, along with Dr. David A. Agard, first demonstrated deconvolution microscopy in the 1980s. Recently, they have developed several new methods of three-dimensional microscopy, including computerized wide-field deconvolution microscopy for multiwavelength fluorescence imaging of living cells, and electron tomography for three-dimensional imaging in the elctron microscope.
VayTek
Specializing in scientific digital imaging and presentation applications, manufactures and distributes deconvolution, three-dimension volume rendering, measurement, and presentation software. Also offered are packages that supply digital control for microscope peripherals.
XCOSM
is anX-Windows interface toComputationalOpticalSectioningMicroscopy (COSM) algorithms for removing out-of-focus light in 3-D volumes collected plane by plane using either widefield or confocal fluorescence microscopy. The interface allows users to view and process multiple 3-D datasets within a simple mouse-driven environment. Although the software was written for a unix based system, it is possible to compile on a PC with linux. Also available on the web site are tutorials, links to other sites, and a list of selected references on deconvolution microscopy.