Darkfield Interactive Tutorials

These Java-based interactive tutorials are designed to complement text pages by allowing students to use a web browser to simulate configuration and operation of a microscope under darkfield illumination. Both the theory and practice of darkfield microscopy are addressed by the tutorials.
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Darkfield Interactive Tutorials

Darkfield Abbe Condensers

Explore how changes in the size of the opaque stop in a simple Abbe darkfield illumination condenser affects light cone shape and numerical aperture in this interactive tutorial.

Darkfield Condenser Adjustment

Visitors and students are allowed to experiment with the adjustable parameters in condenser alignment and configuration for darkfield microscopy in this interactive java tutorial.

Specimen Illumination With Darkfield Condensers

Discover how a darkfield microscopy specimen scatters light into the objective when illuminated with a hollow cone of oblique illumination from a Cardioid darkfield condenser.

Catadioptric Darkfield Reflected Light Objectives

Discover how light that is diffracted, refracted, and reflected by a specimen enters the front lens of a darkfield objective in reflected light microscopy.

Contributing Authors

Mortimer Abramowitz - Olympus America, Inc., Two Corporate Center Drive., Melville, New York, 11747.
Kirill I. Tchourioukanov and Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.