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Fluorochrome Data Tables

The data tables below contain reference information on fluorescence cubes offered by Olympus America, Inc. to support their microscopes. The codes for the excitation columns in Tables 2 are listed in Table 1. Table 2 is a listing of examples of cubes’ configuration of filters and mirrors. The authors wish to caution that we believe this data to be accurate, but it should always be verified by authorized Olympus representatives.

Filter Color Codes
Code
Filter Type
U
Ultraviolet
V
Violet
BV
Blue-Violet
B
Blue
IB
InterferenceBlue Filter
G
Green
IG
Immuno GoldStaining Method
BF
Brightfield,Phase Contrast,and DIC only
Table 1

Table 2 lists the excitation and barrier filters by cube along with the dichroic mirror used in each cube. Use this chart as an aid to guide you in selection of some of the Olympus U-URA cubes for applications listed in our fluorescence and fluorochrome data tables.

Fluorescence Filter Cubes

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Example configuration of Olympus Cubes
Excitation
Type
Excitation Filter(nm)
Dichroic Mirror(nm)
Barrier Filter
U
Wide
330-385
400
420--
U
Narrow
360-370
400
420--
U
Narrow Bandpass
360-370
400
420-460
V
Narrow
400-410
455
455--
BV
Wide
400-440
455
475--
BV
Narrow
420-440
455
475--
B
Wide
450-480
500
515--
IB
Wide, Interference
460-490
505
515IF--
IB
Narrow, Interference
470-490
505
515IF--
G
Super Wide
480-550
570
590--
G
Wide
510-550
570
590--
G
Narrow
530-550
570
590--
IG
Narrow, Interference
520-550
565
580IF--
IY
Wide, Interference
545-580
600
610IF--
Table 2

Contributing Authors

Mortimer Abramowitz - Olympus America, Inc., Two Corporate Center Drive., Melville, New York, 11747.
Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.