Transformed African Green Monkey Kidney Fibroblast Cells (COS-7 Line)

A culture of COS-7 fibroblasts was labeled for mitochondria with MitoTracker Red CMXRos, and for the cytoskeletal filamentous actin network with Alexa Fluor 488 conjugated to phalloidin, a cyclic peptide derived from the toxic death cap fungus (Amanita phalloides). In addition, the fibroblasts were counterstained for DNA in the cell nucleus with Hoechst 33258.