Multiphoton Fluorescence Excitation
Section Overview:
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Multiphoton Fluorescence Excitation
Introduction
Multiphoton microscopy is a research tool that combines the optical techniques of laser scanning microscopy with long wavelength multiphoton fluorescence excitation to capture three-dimensional images of specimens.
Laser Fundamentals
Explore the fundamentals of lasers and how basic laser systems operate throughstimulatedemission, and how they are designed to amplify this form of light to create intense and focused beams.
Laser Systems for Optical Microscopy
The lasers employed in optical microscopy are high-intensity monochromatic light sources, which are useful for a variety of techniques such as scanning confocal fluorescence microscopy.