Multiphoton Fluorescence Excitation

Section Overview:

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Multiphoton Fluorescence Excitation

Introduction

Multiphoton microscopy is a research tool that combines the optical techniques of laser scanning microscopy with long wavelength multiphoton fluorescence excitation to capture three-dimensional images of specimens.

Laser Fundamentals

Explore the fundamentals of lasers and how basic laser systems operate throughstimulatedemission, and how they are designed to amplify this form of light to create intense and focused beams.

Laser Systems for Optical Microscopy

The lasers employed in optical microscopy are high-intensity monochromatic light sources, which are useful for a variety of techniques such as scanning confocal fluorescence microscopy.

Contributing Authors

David W. Piston - Department of Molecular Physiology and Biophysics, Vanderbilt University, 702 Light Hall, Nashville, Tennessee, 37212.
Kenneth R. Spring - Scientific Consultant, Lusby, Maryland, 20657.
John C. Long, Brian O. Flynn, and Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.