Hoffman Modulation Contrast Microscopy
Review Articles
Hoffman Modulation Contrast Basics
The featured section serves as an introduction to the basic concepts in modulation contrast microscopy with numerous illustrations and examples of how the technique is implemented.
Troubleshooting Hoffman Modulation Contrast
Problems with proper slit alignment between the condenser and the objective modulator are the most common source of errors with modulation contrast microscopy.
Interactive Tutorials
Optical Sectioning
Explore this simulated virtual microscope that provides several examples of specimens in optical section while passing through focus using modulation contrast illumination in this interactive tutorial.
Hoffman Modulation Contrast: Slit Alignment
Aligning the condenser slit with the objective modulator plate is one of the most critical aspects of microscope configuration for Hoffman modulation contrast. Practice this simple, yet often misunderstood, alignment technique.
Hoffman Modulation Contrast
Students can discover how rotation of the substage polarizing filter affects image contrast in Hoffman modulation contrast microscopy in this interactive java tutorials.
Digital Image Gallery
Hoffman Modulation Contrast Microscopy Image Gallery
Modulation contrast illumination produces a pseudo three-dimensional effect that can be used in combination with other illumination techniques to achieve spectacular images.