ix3
As the demand for the use of intracytoplasmic sperm injection (ICSI) applications has increased, so has the burden of the microscopy work it involves. Evident’s in vitro fertilization technology (IVF) helps facilitate the ICSI workflow, improving the speed and efficiency of each procedure.
Optimized for a Smoother ICSI Workflow
As the demand for the use of intracytoplasmic sperm injection (ICSI) applications has increased, so has the burden of the microscopy work it involves. Olympus’ in vitro fertilization technology (IVF) helps facilitate the ICSI workflow, improving the speed and efficiency of each procedure.
Easy Oocyte Condition Checks through Spindle Visualization
Olympus’ observation method enables you to easily view the metaphase II (MII) spindle through the microscope oculars with the touch of a button:
- Confirm the oocyte’s maturity to facilitate the timing of the ICSI stage within the in vitro maturation (IVM) process. Never take your eye off the sample with single button operation of the visualization.
- Confirm the spindle’s position relative to the polar body before inserting the pipette to decrease incidences of damage to the oocyte.
- Verify the spindles of vitrified oocytes are stable after thawing. Verification contributes to improved accuracy.
Streamlined ICSI Steps through Motorized Operation
- Switch observation methods instantaneously and adjust the magnification with the press of a button on the integrated hand switch with motorized unit.
- Improve your efficiency as motorized microscope adjustments reduce the number of steps required, enabling you to concentrate on the delicate work of operating the manipulator.
- Manual focusing can be easily performed with a high degree of sensitivity in conjunction with the motorized observation and magnification.
Observation Methods
Relief Contrast
- Unaffected by the polarizing effect of plastic dishes, Olympus’ relief contrast enables 3D imaging of oocytes so you can check the condition of the zona pellucida.
- Correction collars on the 20X and 40X UIS2 objectives can optimize the image acquisition, based on the thickness of the bottom of the dish.
Polarization
- Observe the birefringence of spindles using polarized light.
- Adhesion of granulosa cells to the zona pellicida can be confirmed before the cumulus cells are even stripped.
(Application information and image are courtesy of David F. Albertini, Ph.D., Center for Human Reproduction, NY)
Applied Technologies
Morphological Evaluation of Sperm at High Magnification
Improve the sperm selection process:
- Smooth zooming from 1x, 1.6x, to 2x using the adjustment lever.
- Combine the zoom with the 40X objective lens to observe the morphology and internal structure of the sperm head in detail.
- IMSI DIC option can be added with LUCPLFLN60X objective and IX3-MDIC60.
.
Ease Lab Work with a Comfortable Design
Adjust the oculars to the position that is most convenient and comfortable for you—the vertical setting enables you do a quick check on the oocyte and embryo while standing.
Semi-motorized system
Motorized system
Specifications
| IX83 | IX73 (available as an FDA Class I product in the United States under the name IX73-SC) | ||
| Microscope frame | Optical system | UIS2 optical system | |
| Revolving nosepiece | Motorized sextuple revolving nosepiece (DIC slider attachable), simple waterproof structure |
Motorized sextuple revolving nosepiece (DIC slider attachable), simple waterproof structure） Coded sextuple revolving nosepiece (DIC slider attachable), simple waterproof structure |
|
| Focus | Stroke: 10.5 mm, Minimum increment: 0.01 μm, Maximum nosepiece movement speed: 3 mm/s | Stroke: 10 mm | |
| Intermediate port | 2 ports | ||
| Light path selection | Motorized 0:100/50:50/100:0 (Left side port: BI port) | Manual 0:100/50:50/100:0 (Left side port: BI port) | |
| Transmitted light illuminator |
Pillar tilt mechanism (30 ° inclination angle, with vibration reducing mechanism), Condenser holder (with with 88 mm stroke, refocusing mechanism), Field iris diaphragm adjustable, 4 filter holders Light source: 12 V, 100 W halogen bulb (pre-centered) High color reproductive LED light source |
||
| Observation tube | Widefield (FN 22) |
Widefield tilting binocular Widefield binocular |
|
| Stage | Mechanical stage with left handle | Stage stroke: X: 50 mm x Y: 43 mm, including stage insert plate (ø25 mm), stage insert plate exchangeable (Ø110 mm) | |
| Cross stage with right handle | Stage stroke: X: 50 mm x Y: 50 mm, including stage insert plate (ø25 mm), stage insert plate exchangeable (Ø110 mm) | ||
| Mechanical stage with right handle | Stage stroke: X: 114 mm x Y: 75 mm, stage position locking function | ||
| Condenser | Motorized middle long working distance condenser | NA 0.5, W.D. 45 mm, 4 positions for optical devices (for ø50 mm, Relief Contrast optical devices rotatable) | |
| Mid long working distance relief contrast | NA 0.5, W.D. 45 mm, 4 positions for optical devices (for ø50 mm, Relief Contrast optical devices rotatable) | ||
| Operating environment |
Indoor use Ambient temperature: 5 ºto 40 ºC (41 º to 104 ºF) Maximum relative humidity: 80 % for temperatures up to 31 ºC (88 ºF), decreasing linearly through 70 % at 34 ºC (93 ºF), 60 % at 37 º C (99 ºF), to 50 % relative humidity at 40 ºC (104 ºF) Supply voltage fluctuations: Not to exceed ±10 % of the normal voltage |
||