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Cell Culture Analysis
Assays
Timelapse Images
MSC (Mesenchymal stem cells)
Conditions Vessel: 6 well Interval time: 3 H Measurement period: 7 Days
Time-lapse images
https://adobeassets.evidentscientific.com/content/dam/video/video/library/MSC_6erll_PS0003_00001.mp4
Cell count : 6.24 × 10²
Confluency : 73.37%
Analysis Results
iPSC (Induced pluripotent stem cells)
Conditions Vessel: T25 Interval time: 3 H Measurement period: 20 Days
Time-lapse images
https://adobeassets.evidentscientific.com/content/dam/video/video/library/200619_201B7_43.mp4
Colony count : 6.75 × 10⁰
Confluency : 19.46%
Analysis Results
HEK293 (Human embryonic kidney cells 293)
Conditions Vessel: 12 well Interval time: 3 H Measurement period: 7 Days
Time-lapse images
https://adobeassets.evidentscientific.com/content/dam/video/video/library/HEK293-2-22_43.mp4
Cell count : 2.77 × 10³
Confluency : 87.42%
Analysis Results
HepG2 (Human hepatoma cell line)
Conditions Vessel: 12 well Interval time: 3 H Measurement period: 12 Days
Cell count : 2.62 × 10³
Confluency : 82.45%
Analysis Results
NHEK (Normal human epidermal keratinocytes)
Conditions Vessel: T75 Interval time: 3 H Measurement period: 9 Days
Time-lapse images
https://adobeassets.evidentscientific.com/content/dam/video/video/library/210201_NHEK_P1_43.mp4
Cell count : 2.65 × 10³
Confluency : 45.07%
Analysis Results
HDFa (Normal human dermal fibroblasts)
Conditions Vessel: T75 Interval time: 3 H Measurement period: 7 Days
Time-lapse images
Cell count : 1.06 × 10³
Confluency : 66.00%
Analysis Results
HDFa (Normal human dermal fibroblasts)
Conditions Vessel: 24 well Interval time: 1 H Measurement period: 5 Days
Time-lapse images
Cell count : 1.72 × 10³
Confluency : 90.53%
Analysis Results
MCF7 (Human breast cancer cells)
Conditions Vessel: 96 well Interval time: 1 H Measurement period: 7 Days
Time-lapse images
Cell count : 1.85 × 10³
Confluency : 53.81%
Analysis Results
A549 cells (Adenocarcinomic human alveolar basal epithelial cells)
Conditions Vessel: 12 well Interval time: 1 H Measurement period: 4 Days *Acquire with custom mode
Time-lapse images
Cell count : 3.42 × 10³
Confluency : 70.60%
Analysis Results
Time-lapse images
Cell count : 1.18 × 10³
Confluency : 35.73%
Analysis Results
SH-SY5Y cells (Neuroblastoma)
Conditions Vessel: 12 well Interval time: 1 H Measurement period: 4 Days *Acquire with custom mode
Time-lapse images
Cell count : 2.77 × 10³
Confluency : 61.58%
Analysis Results
Time-lapse images
Cell count : 5.71 × 10²
Confluency : 18.15%
Analysis Results
Scratch Assay
MCF7 (Human breast cancer cells)
Conditions Vessel: 24 well Interval time : 1 H Measurement period: 4 Days
*Acquire with custom mode
Image data courtesy of: ACEL, Inc.
Control
Analysis Results
Sorafenib 10 μM
Analysis Results
Sorafenib 30 μM
Scratch Assay
MCF7 (Human breast cancer cells)
Conditions Vessel: 24 well Interval time : 6 H Measurement period: 3 Days
*Acquire with custom mode
Image data courtesy of : Minsoo Kim, PhD
Associate Professor Medical education center, Graduate School of Medicine, Kyoto University
Migration of ENCDCs from E6 chick midgut
E6 chick midgut
Brief description: The addition of 10 ng/ml GDNF produces a dramatic stimulation of ENCDC migration out of the gut following 32 h in culture.
Image data courtesy of : Ádám Soós, PhD
Department of Anatomy, Histology and Embryology at Semmelweis University
Live cell imaging recordings of E6 chick midgut explants
E6 chick midgut explants
Brief description: ENCDCs migrating out of gut explants in response to 10 ng/ml GDNF cultured for 12 hours (h), return to the explants after DUSP6 is inhibited with BCI for 8 h. Cells proceed to migrate out of the explants again after BCI is removed. Duration: 32 h
Image data courtesy of : Ádám Soós, PhD
Department of Anatomy, Histology and Embryology at Semmelweis University
Primary culture of Bone Marrow Derived MSC
Conditions Vessel: 24 well Interval time : 1 H Measurement period: 11 Days
Image data courtesy of: ACEL, Inc.
NSC sphere
Conditions Vessel: 96Well Round Bottom Interval time: 1 H Measurement period: 3 Days
*Acquire with custom mode
Image data courtesy of : ACEL, Inc.
Spheroid
NSC sphere
Conditions Vessel: 12 well Interval time: 1 H Measurement period: 6 Days
*Acquire with custom mode
Image data courtesy of : ACEL, Inc.
Plant cells
Nicotiana tabacum L.SR1
Conditions Vessel: 12 well Interval time: 1 H Measurement period: 8 Days
*Acquire with custom mode
Image data courtesy of: Shinjiro OGITA, Ph.D.
Director of the Science Center Professor Faculty of Bioresource Sciences Department of Local Resources Prefectural University of Hiroshima
Webinars
*Available in Japanese language only.