CM30 Incubation Monitoring System
The CM30 incubation monitoring system supports observation and data analysis of various cells and vessels. Explore the applications below to learn more about actual experiments and acquired data using CM series systems.
Let us know if you have any questions or would like to request a demo.
Cell Culture Analysis
Assays
Time-Lapse Images
MSC (Mesenchymal Stem Cells)
Vessel conditions: 6 wells, interval time: 3 hours, measurement period: 7 days
Time-lapse images
https://adobeassets.evidentscientific.com/content/dam/video/video/library/MSC_6erll_PS0003_00001.mp4
Cell count: 6.24 × 10²
Confluency: 73.37%
Analysis results
iPSC (Induced Pluripotent Stem Cells)
Vessel conditions: T25, interval time: 3 hours, measurement period: 20 days
Time-lapse images
https://adobeassets.evidentscientific.com/content/dam/video/video/library/200619_201B7_43.mp4
Colony count: 6.75 × 10⁰
Confluency: 19.46%
Analysis results
HEK293 (Human Embryonic Kidney Cells 293)
Vessel conditions: 12 wells, interval time: 3 hours, measurement period: 7 days
Time-lapse images
https://adobeassets.evidentscientific.com/content/dam/video/video/library/HEK293-2-22_43.mp4
Cell count: 2.77 × 10³
Confluency: 87.42%
Analysis results
HepG2 (Human Hepatoma Cell Line)
Vessel conditions: 12 wells, interval time: 3 hours, measurement period: 12 days
Cell count: 2.62 × 10³
Confluency: 82.45%
Analysis results
NHEK (Normal Human Epidermal Keratinocytes)
Vessel conditions: T75, interval time: 3 hours, measurement period: 9 days
Time-lapse images
https://adobeassets.evidentscientific.com/content/dam/video/video/library/210201_NHEK_P1_43.mp4
Cell count: 2.65 × 10³
Confluency: 45.07%
Analysis results
HDFa (Normal Human Dermal Fibroblasts)
Vessel conditions: T75, interval time: 3 hours, measurement period: 7 days
Time-lapse images
Cell count: 1.06 × 10³
Confluency: 66.00%
Analysis results
HDFa (Normal Human Dermal Fibroblasts)
Vessel conditions: 24 wells, interval time: 1 hour, measurement period: 5 days
Time-lapse images
Cell count: 1.72 × 10³
Confluency: 90.53%
Analysis results
MCF7 (Human Breast Cancer Cells): Example 1
Vessel conditions: 24 wells, interval time: 1 hour, measurement period: 5 days
Time-lapse images
Cell count: 1.85 × 10³
Confluency: 53.81%
Analysis results
A549 cells (Adenocarcinomic Human Alveolar Basal Epithelial Cells)
Vessel conditions: 12 wells, interval time: 1 hour, measurement period: 4 days. Acquired with custom mode.
5-FU Untreated Group
Time-lapse images
Cell count: 3.42 × 10³
Confluency: 70.60%
Analysis results
5-FU Treated Group
Time-lapse images
Cell count: 1.18 × 10³
Confluency: 35.73%
Analysis results
SH-SY5Y Cells (Neuroblastoma)
Vessel conditions: 12 wells, interval time: 1 hour, measurement period: 4 days
Acquired with custom mode.
6-OHDA Untreated Group
Time-lapse images
Cell count: 2.77 × 10³
Confluency: 61.58%
Analysis results
6-OHDA Treated Group
Time-lapse images
Cell count: 5.71 × 10²
Confluency: 18.15%
Analysis results
Scratch Assay
MCF7 (Human Breast Cancer Cells): Example 2
Vessel conditions: 24 wells, interval time: 1 hour, measurement period: 4 days
Acquired with custom mode.
Image data courtesy of ACEL, Inc.
Analysis results
Sorafenib 10 μM
Analysis results
Sorafenib 30 μM
Analysis results
Scratch Assay
MCF7 (Human Breast Cancer Cells): Example 3
Vessel conditions: 24 wells, interval time: 6 hours, measurement period: 3 days
Acquired with custom mode
Image data courtesy of Minsoo Kim, PhD
Associate Professor, Medical Education Center, Graduate School of Medicine, Kyoto University
Migration of ENCDCs from E6 Chick Midgut
E6 Chick Midgut
The addition of 10 ng/ml GDNF produces a dramatic stimulation of ENCDC migration out of the gut following 32 hours in culture.
Image data courtesy of Ádám Soós, PhD
Department of Anatomy, Histology and Embryology at Semmelweis University
Live-Cell Imaging of E6 Chick Midgut Explants
E6 Chick Midgut Explants
ENCDCs migrating out of gut explants in response to 10 ng/ml GDNF (cultured for 12 hours) (h) return to the explants after DUSP6 is inhibited with BCI for 8 h. Cells proceed to migrate out of the explants again after BCI is removed. Duration: 32 h.
Image data courtesy of Ádám Soós, PhD
Department of Anatomy, Histology and Embryology at Semmelweis University
MC-MSC
(Primary Culture of Bone Marrow-Derived Mesenchymal Stem Cells)
Vessel conditions: 24 wells, interval time: 1 hour, measurement period: 11 days
Image data courtesy of ACEL, Inc.
NSC Sphere
Vessel conditions: 96 wells, round bottom, interval time: 1 hour, measurement period: 3 days
Acquired with custom mode
Image data courtesy of ACEL, Inc.
Spheroid
NSC sphere
Vessel conditions: 12 wells, interval time: 1 hour, measurement period: 6 days
Acquired with custom mode
Image data courtesy of ACEL, Inc.
Plant Cells
Nicotiana Tabacum L.SR1
Vessel conditions: 12 wells, interval time: 1 hour, measurement period: 8 days
Acquired with custom mode
Image data courtesy of Shinjiro Ogita, PhD
Director of the Science Center, Professor Faculty of Bioresource Sciences, Department of Local Resources, Prefectural University of Hiroshima
