CM Incubation Monitoring System supports observation and data analysis of various cells and various vessels. Here, we introduce actual experiments and acquired data using the CM20 and CM30.
If you have any questions or would like to request a demo, let us know!
Cell Culture Analysis
Assays
Timelapse Images
MSC (Mesenchymal stem cells)
Conditions Vessel: 6 well Interval time: 3 H Measurement period: 7 Days
iPSC (Induced pluripotent stem cells)
Conditions Vessel: T25 Interval time: 3 H Measurement period: 20 Days
HEK293 (Human embryonic kidney cells 293)
Conditions Vessel: 12 well Interval time: 3 H Measurement period: 7 Days
Cell count : 2.77 × 10³
Confluency : 87.42%
Analysis Results
HepG2 (Human hepatoma cell line)
Conditions Vessel: 12 well Interval time: 3 H Measurement period: 12 Days
Cell count : 2.62 × 10³
Confluency : 82.45%
Analysis Results
NHEK (Normal human epidermal keratinocytes)
Conditions Vessel: T75 Interval time: 3 H Measurement period: 9 Days
Cell count : 2.65 × 10³
Confluency : 45.07%
Analysis Results
HDFa (Normal human dermal fibroblasts)
Conditions Vessel: T75 Interval time: 3 H Measurement period: 7 Days
HDFa (Normal human dermal fibroblasts)
Conditions Vessel: 24 well Interval time: 1 H Measurement period: 5 Days
MCF7 (Human breast cancer cells)
Conditions Vessel: 96 well Interval time: 1 H Measurement period: 7 Days
A549 cells (Adenocarcinomic human alveolar basal epithelial cells)
Conditions Vessel: 12 well Interval time: 1 H Measurement period: 4 Days *Acquire with custom mode
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SH-SY5Y cells (Neuroblastoma)
Conditions Vessel: 12 well Interval time: 1 H Measurement period: 4 Days *Acquire with custom mode
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Scratch Assay
MCF7 (Human breast cancer cells)
Conditions Vessel: 24 well Interval time : 1 H Measurement period: 4 Days
*Acquire with custom mode
Image data courtesy of: ACEL, Inc.
Control
Analysis Results
Sorafenib 10 μM
Analysis Results
Sorafenib 30 μM
Analysis Results
Scratch Assay
MCF7 (Human breast cancer cells)
Conditions Vessel: 24 well Interval time : 6 H Measurement period: 3 Days
*Acquire with custom mode
Image data courtesy of : Minsoo Kim, PhD
Associate Professor Medical education center, Graduate School of Medicine, Kyoto University
https://adobeassets.evidentscientific.com/content/dam/video/videolibrary/videos/20210311_scratch_MB231_MCF7_PS0001_00001.mp4
https://adobeassets.evidentscientific.com/content/dam/video/videolibrary/DEV201090_MovieS1.mp4?rev=E56C
Migration of ENCDCs from E6 chick midgut
E6 chick midgut
Brief description: The addition of 10 ng/ml GDNF produces a dramatic stimulation of ENCDC migration out of the gut following 32 h in culture.
Image data courtesy of : Ádám Soós, PhD
Department of Anatomy, Histology and Embryology at Semmelweis University
Live cell imaging recordings of E6 chick midgut explants
E6 chick midgut explants
Brief description: ENCDCs migrating out of gut explants in response to 10 ng/ml GDNF cultured for 12 hours (h), return to the explants after DUSP6 is inhibited with BCI for 8 h. Cells proceed to migrate out of the explants again after BCI is removed. Duration: 32 h
Image data courtesy of : Ádám Soós, PhD
Department of Anatomy, Histology and Embryology at Semmelweis University
Primary culture of Bone Marrow Derived MSC
Conditions Vessel: 24 well Interval time : 1 H Measurement period: 11 Days
Image data courtesy of: ACEL, Inc.
NSC sphere
Conditions Vessel: 96Well Round Bottom Interval time: 1 H Measurement period: 3 Days
*Acquire with custom mode
Image data courtesy of : ACEL, Inc.
Spheroid
NSC sphere
Conditions Vessel: 12 well Interval time: 1 H Measurement period: 6 Days
*Acquire with custom mode
Image data courtesy of : ACEL, Inc.
Plant cells
Nicotiana tabacum L.SR1
Conditions Vessel: 12 well Interval time: 1 H Measurement period: 8 Days
*Acquire with custom mode
Image data courtesy of: Shinjiro OGITA, Ph.D.
Director of the Science Center Professor Faculty of Bioresource Sciences Department of Local Resources Prefectural University of Hiroshima
