CM30 Cell Culture Monitoring System

Name: CM30 Incubation Monitoring System | Evident Scientific | Olympus
Brand: Evident
SKU: cm30
Availability: InStock

Remotely monitor, analyze, and share your cell cultures’ health, cell count, and confluency using the reliable quantitative data provided by the automated CM30 incubation monitoring system. The system enables label-free observation, reduces the risk of damage to your cultures, and standardizes your culture workflow.

CM30 Cell Culture Monitoring System

Control Your Process with Smart Cell Culture Monitoring

Culturing cells can be costly, complicated, and time-consuming. With the CM30 incubation monitoring system, there’s a simple way to improve your culture process.

Application Gallery

The CM incubation monitoring system supports the observation and data analysis of various cells and vessels.

See the application gallery

Improve the Cell Culture Process—Label-Free, Quantitative Results

Label-Free Cell Monitoring

With the CM30 system, there is no need to stain the cultures to monitor their status. It acquires quantitative data from label-free cells, reducing the chance of harm to your cultures.

Multipoint Cell Culture Monitoring

The system automatically scans multiple points in your culture vessel, providing periodic quantitative data about the health and confluency of your cultures.

Label-free detection

Multipoint Cell Culture Monitoring

Label-free quantification

Consistent Results Throughout Your Lab

Customize the Analysis Parameters to Suit Your Experiment

Consistent Results Throughout Your Lab

Constant Analysis Parameters

The CM30 system uses image analysis technology based on machine learning to continuously measure and analyze the images acquired. Constantly visualizing the culture status as a quantitative value eliminates factors that cause variations in cell checks and contributes to the reproducibility and consistency of experiments.

Compare Data Across Multiple Samples

Using multiple heads helps you maintain a control sample while multiple test protocols run at the same time. Share your data with team members or compare it to past data.

Customize the Analysis Parameters to Suit Your Experiment

The CM30 system automatically performs confluency, cell counts, and colony counts from acquired images. You can configure the system’s analysis parameters to suit each cell culture’s conditions, such as cell type, culture conditions, or drug administered. Knowing the stepwise cell culture status at each timing improves the accuracy of the experiment.

Cost Effective

Save Time with Automation

Improve your traditional microscopy-based workflow and get more accurate results in less time. By automating cell culture monitoring using the CM30 system, you can expand your research and use your time more effectively

No Need to Enter the Clean Room for Monitoring

Every time you enter the clean room, there is an operational cost for consumables and measurements. Now, you no longer constantly need to check cell culture status outside the incubator. You can reduce your costs by remotely checking the status of your cultures from outside the lab.

Accurately Time Cell Passage

Time cell passages consistently and without the subjectivity associated with manual counting. Based on your set standardized parameters, the software can indicate when your cells are ready for passage, helping prevent failures.

Cost Effective

Testimonials

Dr. Tadayuki Tsujita

Dr. Tadayuki Tsujita
Saga University
Biochemistry Lab, Faculty of Agriculture

One of our research themes is developing novel hypoxic stress protection compounds. In order to validate the compound treatment, we were struggling to observe cell morphology and growth data without disturbing the continuous low oxygen environment. This is critical to analyzing super-short half-life proteins. We tried to several equipment combinations to solve this difficulty.

In this time, we selected the CM30 system because it is compact and can be installed in our hypoxic incubator. It enables us to obtain experimental data such as serial dilution in multi-well plates in real time. The CM30 does not required mechanical moving to monitor all of the wells while maintaining hypoxic conditions, which had been difficult in the past. In addition, by connecting online, we can remotely observe the state of the cells from the outside, which we expect will be useful for troubleshooting.

Takanori Takebe, Dr.

Takanori Takebe, Dr.
Institute of Science Tokyo， Graduate School of Medical and Dental Sciences， Medical and Dental Sciences, Division of Advanced Therapeutic Sciences

Numerous researchers may be curious about the biological mechanisms involved in the long-term culturing required for the formation of stem cells and organoids within an incubator. I was honestly surprised that such a simplistic microscope is poised to address these questions as this CM20* can visualize spheroid formation and organoids cultured in an extracellular matrix gel with amazing contrast. I believe that CM20 can be a transformative tool in a wide range of cell biology experiments.

Learn more about Dr. Takebe’s research

Erik A. Blackwood, Ph.D.

Erik A. Blackwood, Ph.D.
Translational Cardiovascular
Research Center University of Arizona

Dr. Erik Blackwood and his team have implemented the CM20* incubation monitoring system to optimize their cell culture monitoring process. They use the system to determine when cell lines are ready for their preclinical trials in rodent models for heart failure. Their approach focuses on identifying small molecule drug-based therapies, but also on gene therapy approaches. The count and confluency calculation capabilities of the CM20* system enable the researchers to monitor the effects of these therapeutics on iPS (induced pluripotent stem) cell lines in real time while the cultures remain safely in the incubator. Utilizing the system’s automated data gathering functions also increases their workflow efficiency.

Learn how Dr. Blackwood and his team improved their cell culture workflow with the CM20* monitoring system.

Watch

Mr. Yoshihito Tachi

Interstem Co., Ltd.
Mr. Yoshihito Tachi

I’m in charge of research and development of an autologous chondrocyte kit and conduct cell culture under various conditions when examining the culture protocol. I previously used a microscope to observe the conditions of cultured cells, but I can only make a qualitative evaluation with this method. Another challenge is that the analysis results vary depending on the experience and subjectivity of the worker. In contrast, the Olympus CM20* system can count cells and measure confluency with the same constant analysis parameters for highly reproducible and consistent analysis results. We can compare data between experiments when changing culture conditions, compare it with past data, and easily share it within the team— all of which can help us improve development efficiency. We’re also considering using it as an educational tool. By seeing the indicators, such as adhesion rate, uniformity, and proliferative ability, we can better evaluate the skills of workers.

Applied Technologies

Leave Your Cultures in the Incubator

The monitor lets you track the health of cell cultures without removing them from the incubator, reducing the risk of contamination or damage from temperature changes and vibration. Its unique design enables you to fit up to four head units inside a standard incubator for greater efficiency.

Realized a Compact Design by Integrating the Optical Systems

Flat Design Accommodates Various Vessels

Evident's epi-oblique optical system enables the CM30 incubation monitoring system to have a compact, flat design that accommodates most standard cell culture vessels. Simply place the culture vessel you normally use on the CM30.

Learn more about epi-obique optical system

AI-Driven Automated Cell Analysis

By training the system to distinguish between the cells and background using machine learning, it can automatically determine confluency.

Learn more

Reproducible, Comparable Data

The CM30 makes it easy to store and share detailed quantitative records of cell growth and health.

Graph confluency data to look at cell counts and colony analysis in chronological order to see how they’ve changed over time
The comparison function makes it simple to compare current data with past results or to compare between wells
Enables you to quickly calculate cell characterization data, such as doubling time and the population doubling level (PDL)

Customize the Settings to Meet Your Needs

Monitoring points

Set the monitoring points in the vessel and well plate anywhere you would like, as shown in the vessel on the right. By specifying the rows and columns, you can set multiple points at once.

Focus position

Freely set the focus position for each monitoring point.

Monitoring time

Freely set the start time and interval time.

Customize the Settings to Meet Your Needs

Application Program Interface (API) to Freely Control the Acquisition Parameters (β ver.)

Application Programming Interface (API)to Freely Control the Acquisition Parameters (β ver.)

Remote, independent control of the CM30 head to:

Set key parameters

Illumination
Stage (XY, Z）
Focus（autofocus/ manual focus）
Exposure and image capture

Image Capture and Acquisition

Static image capture
Live preview
Acquire and delete image data
Obtain the list of images

Download β version software (Windows)

Download β version software (Linux)

IQ/OQ Validation Service

Our world-class support team can help you integrate the CM30 incubation monitoring system into your lab. IQ/OQ validation services are also available.

FAQ

Functions & Specifications

Can the CM30 incubation monitoring head be installed in our current incubator?

With its compact design (73 mm (2.9 in.) tall, 212 mm (8.3 in.) wide, and 360 mm (14.2 in.) long) and weighing only approximately 3.1 kg (6.8 lb), the CM30 fits easily in most standard-sized incubators.

Is the head compatible with general cell culture containers?

Yes. The CM30 is compatible with the following vessels:

Petri dishes (90 mm and 100 mm)
Microplates (6 well, 12 well, 24 well, 48 well, 96 well)
Flasks (T25, T75, T80, T150, T175, T225)
Multi-layer flask

What kinds of cells can the system observe?

It’s designed to observe adherent cells, such as MSC and iPS cells.

What can it measure?

It estimates cell population and confluency from an acquired image. For samples that form a colony, it estimates the number of colonies and confluency.

Will there be any damage to the cells?

The system is designed to record quantitative data from inside an incubator, minimizing the chances of damaging your cell cultures.

Can you observe fluorescence?

It cannot observe fluorescence. We offer CKX53 inverted microscopes for fluorescence observation.

How far can you remotely monitor?

It depends on your router specifications and usage environment. If you want to extend the communication range, we recommend using a repeater.

Can the CM30 incubation monitoring head be sterilized or disinfected?

Yes. The head can be sterilized using:

Hydrogen peroxide (H ₂ O ₂ ) gas sterilization
Autoclave sterilization (for vessel holder and sponge rubber only)
UV ray sterilization

Disinfected using:

Peracetic acid disinfection (cold sterilant)
Alcohol disinfection

Support

What is the support system after purchase?

It depends on your region. Please contact your local sales representative.

Is it possible to correspond when IQ / OQ validation is required?

Yes. For details, please contact your local sales representative.

Can the CM30 incubation monitoring system be connected to a network?

Yes. It is possible after building the connection environment at the customer's responsibility. However, we do not guarantee the network connection in the customer's environment. When connecting to the network, it is necessary to set the station PC. Learn more from here.

Release Notes

CM30 Release Notes

CM30 Version 2.2.7

The CM30 incubation monitoring system has been updated with bug fixes and feature enhancements. Please find the details of the updates below.

New Features/Improvements

Removed the Olympus logo and replaced it with the Evident logo.
Added a feature that allows automatic termination by setting the imaging period when creating a project.
Enabled selection of high-quality and low-quality imaging in Custom Project Setting.
Fixed an issue where the log download feature did not work.

CM30 Version 2.2.6

The CM30 incubation monitoring system has been updated with bug fixes and feature enhancements. Please find the details of the updates below.

New Features/Improvements

Updated PC configuration (CPU, OS, BIOS) to support the latest environment.
Improved the Vessel Create function so that Vessels with the same name are now correctly displayed and managed in the list.
Refined the behavior after navigating to the Vessel Create screen to improve overall stability.
Updated the user manual to reflect the latest functions and operating procedures. The manual can be accessed from the Help menu.
Increased the maximum number of concurrent users from 4 to 50.

CM30 Version 2.2.5

The CM30 incubation monitoring system has been updated with bug fixes and feature enhancements. Please find the details of the updates below.

New Features/Improvements

Updated PC configuration (CPU, OS, BIOS) to support the latest environment.
Disabled OLSC service settings in the configuration screen following the discontinuation of the service.
Optimized the display of the Doubling Time label, improving data visibility.
Adjusted the behavior of well plate well number pop-ups to ensure consistent display.
Improved Z-position setting accuracy in Custom Mode MF, enhancing positioning precision during project execution.
Optimized the GUI layout of the Area Select screen when selecting 48-well plates or higher, enhancing visibility and usability.
Refined log management functionality to maintain output within appropriate limits, improving performance and system management.
Updated the user manual to reflect the latest features and operating procedures. The latest version is available through the help menu.

CM30 Version 2.2.4

The CM30 incubation monitoring system has been updated with bug fixes and feature enhancements. Please find the details of the updates below.

New Features/Improvements

To address vulnerabilities in Java 7 and Java 8 (JDK 1.7 and JDK 1.8), the system has been updated to Java 21, improving security and performance.
The user manual has been revised to correct minor inaccuracies. The latest version of the manual is available through the help menu.
Fixed an issue where loaded Colony analysis parameters could not be saved.
Resolved an issue where the UI would freeze when selecting "ME" in the "Select Passage" option of the Comparison feature.

Improvements

Fixed a problem with ‘the Z focus position at image acquired in the project execution’ being different from 'the Z focus position in the initial settings', When the manual focus is selected in custom mode.

CM20H API Release Notes

CM20H API Version 1.6.3

Application Program Interface (API) which allows you to freely control the monitoring head CM20H/CM30H from your PC without the incubation monitoring station. Release note in the download package provides further details.