FLUOVIEW FV4000MPE Multiphoton Microscope
Transform your images with the FLUOVIEW™ FV4000MPE multiphoton laser scanning microscope. Advanced imaging technology reveals the details and dynamics of your samples while providing quantitative image data that can enhance your experiments. With our breakthrough SilVIR detector at the core of the system, achieve much lower noise, higher sensitivity, and improved photon resolving capabilities. Coupled with high speed to capture fast dynamics, the system empowers demanding research applications.
- Product Status: This product and earlier FV series systems FV1200MPE and FVmpe-rs have been replaced by the FV5000MPE.
Transforming Precision Imaging
In-Depth Precision for In Vivo Experiments
Transform your images with the FLUOVIEW™ FV4000MPE multiphoton laser scanning microscope. Advanced imaging technology reveals the details and dynamics of your samples while providing quantitative image data that can enhance your experiments. With our breakthrough SilVIR detector at the core of the system, achieve much lower noise, higher sensitivity, and improved photon resolving capabilities. Coupled with high speed to capture fast dynamics, the system empowers demanding research applications.
Experience the system’s innovations, including:
- Acquire accurate, quantitative image data from the macro scale to subcellular structures
- Gentler, higher-quality live in vivo imaging
- Obtain more information from a single multicolor image
- Monitor neuron and other essential dynamics with high-speed imaging
- Modular design to meet your ever-changing needs
Multiphoton Imaging Made Simple
The FV4000MPE microscope uses our advanced, silicon-based SilVIR™ detector that makes it easier than ever to acquire precise, reproducible data.
SilVIR Next-Generation Detector Technology
The SilVIR detector combines two advanced technologies—a silicon photomultiplier (SiPM) and our patented* fast signal processing design.
- High dynamic range
- Low noise
- No degradation of sensitivity
- Less sensitivity variation among other detectors
*Patent number US11237047
Learn more about SilVIR detector
3D image of a live mouse brain from the surface to 900 µm deep using a TruResolution objective. The brighter neural cell body did not saturate thanks to the SilVIR detector’s high dynamic range.
Sample courtesy of: Aoi Gohma and Atsushi Miyawaki, RIKEN CBS-EVIDENT Open Collaboration Center.
Outstanding Multiphoton Images at Depth
The FV4000MPE microscope equipped with a TruResolution objective can efficiently excite fluorochromes deep in your sample by concentrating excitation laser power to achieve a brighter image. The advanced nondescanned (NDD) SilVIR detector has a larger beam diameter to better collect scattered fluorescence emissions from deeper in samples. The upgraded non-descanned detector (NDD) can be configured for a maximum of six channels for multicolor multiphoton imaging. Resonant scanner enables you to acquire large-volume 3D images quickly.
Stitched image of a cleared whole brain captured using 10X objective lens and resonant scanner (512x512 pixels. Z 501 slices, 225 positions). New resonant scanner has the ability to acquire equivalent quality images to galvo scanner in less than a third of the time.
Sample courtesy of: Tetsushi Hoshida and Atsushi Miyawaki, Laboratory for Cell Function Dynamics, RIKEN CBS.
Gentler, High-Speed Time-Lapse Imaging
- Upgraded resonant scanner enables your to acquire high-resolution images over a wider area at high speed
- The SilVIR detector’s high sensitivity delivers a better signal-to-noise ratio than other detector types, producing higher-quality images at higher speeds, enabling you to precisely acquire the dynamics of live cells
https://adobeassets.evidentscientific.com/content/dam/video/video/library/iGluSnFR_BOCC_movie.mp4
Visualization of glutamatergic synaptic inputs in vivo mouse FrA cortex using iGluSnFR.Courtesy of Katsuya Ozawa and Akiko Hayashi-Takagi, Multi-Scale Biological Psychiatry, RIKEN CBS.
https://adobeassets.evidentscientific.com/content/dam/video/video/library/jRGECI1a_BOCC_movie.mp4
In vivo Ca2+ imaging in mouse cortical neurons using jRGECO1a.Courtesy of Katsuya Ozawa and Akiko Hayashi-Takagi, Multi-Scale Biological Psychiatry, RIKEN CBS.
Support and Service You Can Count On
The FV4000MPE system is easy to maintain.
- Semiconductor-based SilVIR detector is stable and durable
- Laser power monitor continuously checks the illumination conditions and makes adjustments to maintain the same laser power
- System admin can view log files to keep track of the service maintenance schedule
We stand behind our products with a commitment to fast service and technical support to help our customers achieve their goals. We offer various support plans to keep your microscope running at peak performance at a predictable cost as well as remote support options, so you don’t need to wait for an engineer or specialist to visit if you’re having an issue.
Configurations
Many Possibilities, One System
Depending on your application, you can choose between upright, gantry, or inverted microscope frames.
The FV4000MPE microscope is engineered to be modular, making it easy for you to configure the system based on your applications and budget. You can start with a standard FV4000MPE and easily upgrade to a combo system by adding the SPE module as your research changes.
Add Your Own Custom Components
Take your system customization to the next level by incorporating your own components. For instance, you have the option to add a custom laser to the available port, which allows for advanced techniques such as three-photon or imaging methods.
Your science is constantly evolving and requires dynamic solutions to meet the changing demands on your imaging systems. To expand your research capabilities, FV4000/FV4000MPE specialized solutions* increase the capabilities of our standard systems to meet your research applications and requirements.
*Not available in some countries or regions.
Specifications
Excitation wavelength: 690 nm–1300 nm
Four-axis auto alignment, auto beam expander
Resources
Application Notes
Insights
Videos
In vivo Ca2+ imaging in mouse cortical neurons using jRGECO1a
https://adobeassets.evidentscientific.com/content/dam/video/video/library/jRGECI1a_BOCC_movie.mp4
Visualization of glutamatergic synaptic inputs in vivo mouse...
https://adobeassets.evidentscientific.com/content/dam/video/video/library/iGluSnFR_BOCC_movie.mp4