FV5000 Confocal Laser Scanning Microscope

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Simply Powerful Imaging: Faster, Smarter, Clearer

The FLUOVIEW FV5000 is a platform built for every dimension of discovery. From crisp, photon-level quantitation at the surface to deep, multiphoton imaging in thick, living samples, the FV5000 captures biology at every scale.

SilVIR™ detectors deliver exceptional sensitivity, a wide dynamic range, and photon-level precision, while 2K resonant and 8K galvo scanners freeze motion in real time for exceptional clarity. Smart automation simplifies setup and workflow and ensures consistent, reproducible results.

Combining advanced detection, intelligent design, and intuitive operation, the FV5000 makes powerful imaging more accessible than ever, helping researchers capture more detail, more reliably, in less time.

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Two Scanners, One Workflow: High-Density Sampling and High-Speed Imaging. No Compromises.

Whether you need high-speed series or pixel-dense maps, the FV5000 adapts. Our resonant scanner captures fast cellular dynamics at full clarity across a 20 mm FOV with little to no averaging, giving you high-quality, high-SNR raw images, in up to 438 FPS.

Switch to 8K × 8K galvo scanning to image large areas at high spatial resolution with ultra-fast pixel dwell times as short as 0.2 µs. Achieve up to 120 nm XY resolution across six spectral channels with high NA objectives and FV-OSR software—no additional hardware required.

The result: fewer compromises and faster time to data.

Stitched mouse brain slice cleared with SeeDB2. EYFP is expressed in cortical layer 5 pyramidal neurons in Thy1-YFP-H transgenic mice, acquired with a LUPLAPO25XO objective lens and resonant scanner. Sample courtesy of: Drs. Satoshi Fujimoto and Takeshi Imai, Graduate School of Medical Sciences, Kyushu University.

Biology in Motion, from Cells to Embryos

See biology in motion, in detail. From FRAP to ablation, SilVIR quantitative detection and fast scanning allow you to capture weak signals with minimal averaging, delivering high SNR at real-time frame rates.

In living samples, precise stimulation enables repeatable, targeted perturbations, so you can track repair, migration, and signaling over time. A wide linear dynamic range helps prevent saturation, keeping time-lapse data analysis-ready.

Time-lapse sequence in a living zebrafish embryo showing the repair response after localized multiphoton ablation of microtubules (green). Sample courtesy of: Soraya Villaseca, PhD , Department of Physiology, Development and Neuroscience, University of Cambridge.

End-to-End Simplicity with FLUOVIEW Smart™ Software

Inspired by real researchers tackling the challenges of science, the FV5000 is transforming confocal imaging, making it smarter and faster.

• Smart Sample Search quickly locates your sample in XY and Z
• Smart Auto Laser Power Adjustment uses AI to optimize signal-to-noise across power setting
• Intuitive interface captures complex multidimensional images in just a few clicks
• Intelligent Shading Correction automatically creates seamless, high-quality stitched images

Note: FLUOVIEW Smart is available with FV5000 inverted configurations. FLUOVIEW Smart is not compatible with gantry, upright, or MPE configurations.


Read more about how Evident is revolutionizing confocal microscopy with FLUOVIEW Smart’s intuitive AI-enhanced workflows.

Intelligent Shading Correction

For edge-to-edge clarity and precision, automatically create seamless, high-quality stitched images with Intelligent Shading Correction.

Left: Without Intelligent Shading Correction. Right: With Intelligent Shading Correction. Intelligent Shading Correction automatically compensates for uneven illumination across the field of view to generate stitched images without manual adjustment, improving efficiency and consistency in large-area imaging.

Image Segmentation Powered by Deep Learning

Go Beyond Conventional Thresholds

Traditional intensity-based thresholding can be slow, inconsistent, and highly sensitive to sample conditions.

TruAI image segmentation uses deep learning to recognize subtle patterns and faint signals that conventional methods miss, enabling accurate, reproducible segmentation of weakly labeled structures and complex tissues.

Clearly distinguish cells—and quickly find answers.

Spheroid imaging and analysis with TruAI. Left: Without TruAI. Right: With TruAI. TruAI segments and classifies cells (images on the right), even at high-penetration depths when the nuclei DAPI signal becomes weaker due to scattering.

Engineered for Long-Term Precision, Built to Last

The FV5000 is engineered for long-term precision and adaptability, delivering the reliability and flexibility your science demands. Configure your system for today’s needs and expand effortlessly as your research evolves.

Add detectors, cameras, or lasers as workflows advance, or upgrade to multiphoton imaging with the MPE module, enabling single- and multiphoton acquisition as well as second and third harmonic generation. Smart hardware and software continuously monitor and optimize imaging performance, ensuring consistent, reproducible results. And with Evident’s global service and support network, every system is built to deliver lasting confidence and uptime.

Built for Reproducibility

The FV5000 maintains measurement precision through active system monitoring. The Laser Power Monitor (LPM) ensures consistent laser output across sessions, allowing different users to acquire images under identical conditions, even days or weeks apart. This stability supports the reproducibility required for quantitative and longitudinal studies.

To further safeguard performance, the Microscope Performance Monitor (MPM) automatically evaluates system sensitivity and imaging consistency. It detects deviations early, helping researchers maintain confidence in their results and ensuring every dataset reflects true experimental conditions.

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Support and Service You Can Count On

When it comes to protecting your investment and the integrity of your research, your needs come first. We stand behind our products with a commitment to prompt service and technical support to help you achieve your goals.

Available in three convenient tiers—Maintenance, Protection, and Performance Plus—our FV5000 Service Plans* include priority support to help minimize downtime, regular scheduled maintenance to keep your equipment in peak condition, predictable repair costs to eliminate unplanned expenses, and direct, efficient solutions when you need them most.

^{*Regional variations in service offerings may apply.}