IXplore IX85 SpinSR Super Resolution Microscope System
Capture ultra-small cellular dynamics in stunning detail with the IXplore™ IX85 SpinSR super-resolution microscope system. Designed for prolonged cell viability in time-lapse experiments, the IX85 SpinSR combines fast image acquisition, advanced algorithms, and XY resolution down to 120 nm.
Stunning Detail, Faster Discovery: IXplore™ IX85 SpinSR Microscope
Capture ultra-small cellular dynamics in stunning detail with the IXplore IX85 SpinSR super-resolution microscope system.
- Discover more with high-speed imaging in resolution down to 120 nm
- Designed for prolonged cell viability in long-term time-lapse experiments
- Powered by Yokogawa spinning disk technology
- Enhanced with the IXplore IX85 platform and Evident’s TruSight™ SR super-resolution algorithm
- Learn how the TruSight™ SR algorithm achieves super-resolution imaging
30 µm maximum intensity projection of a mouse brain section, GFP.
Scale bar 2 um. Sample courtesy of SunJin Lab Co., Taiwan.
Rapid Super Resolution for Live Cells
With fast image acquisition and advanced algorithms, the IXplore IX85 SpinSR makes capturing super-resolution details even easier so you can discover new insights faster. Observe dynamic subcellular processes with fast, gentle live-cell imaging.
Confocal image
Super-resolution image
NIH3T3 cells labeled with Hoechst (blue), β-tubulin–AF555 (green), CPCA–HSP60–AF647 (magenta), and fibrillarin–AF568 (gray). Scale bar: 5 µm. Sample courtesy of EnCor Biotechnology Inc.
Synchronized Imaging at High Speed
Featuring a fast frame rate for super-resolution imaging, the IXplore IX85 SpinSR rapidly acquires dynamic cellular events at the nanoscale with XY resolution down to 120 nm. Capture ultra-small cellular dynamics by imaging at speeds up to 200 fps at full frame.
Our real-time controller works as the system’s conductor, ensuring imaging is coordinated with microsecond accuracy. It delivers coordinated imaging even at the highest speeds, reducing the risk of sample damage with shorter exposures.
Live super-resolution imaging of LC3-EGFP expressing U2OS cells.
Acquired with the UPLSAPO100XS objective lens.
Bright Super-Resolution Images of Live Cells
The high-sensitivity model equipped with a SoRa disk realizes brighter super resolution image acquisition thanks to the spinning disk with a microlens in the confocal pinhole. Each confocal pinhole enables you to image with lower laser power, reducing photobleaching and phototoxicity in your sample while enabling bright super-resolution images.
Image clarity depends on how the sample is illuminated and how efficiently the emitted light is collected. In a standard spinning disk confocal system, some light passes through the pinhole but may not be clearly resolved if it is off center. The SoRa disk addresses this by using microlenses to redirect each point of light toward the center of the pinhole, resulting in sharper, brighter images. This approach enhances resolution to nearly the best possible level—like what could be achieved with a theoretically infinitesimal pinhole.
Enhance Image Resolution with One Click
Evident’s TruSight SR super-resolution algorithm enhances image resolution down to 120 nm with one click. It automatically optimizes the image processing filter based on the signal-to-noise ratio, enabling appropriate processing for live-cell imaging, even in dim fluorescence conditions. Its 3D algorithm supports organoid research by delivering clear and precise super-resolution results, even deep inside your sample.
Point spread function (PSF) measured using 100 nm beads by an UPLSAPO100XS (NA 1.35) objective lens with 488 nm laser excitation.
Quantitative Super-Resolution Data
The TruSight SR algorithm preserves the original image’s intensity levels, enabling you to analyze and compare the brightness of samples in super-resolution images. These stable results support quantitative analysis.
Original image
With TruSight SR
Intensity histogram of original and TruSight SR images
Discover More on One Platform
As the needs of your facility grow, the IXplore IX85 SpinSR can be expanded to meet them. With flexible frame customization options, this super-resolution system adapts to a wide range of applications.
- Supports a wide wavelength range from 405 to 640 nm, offering the flexibility to use the ideal wavelength for your fluorophores
- Compatible with incubation systems and environmental controls for stable long-term experiments
- Versatile imaging modes: Switch between widefield, confocal, and super-resolution imaging with one click
Seamless Workflows
The IXplore IX85 SpinSR is designed to drive efficiency with easy workflows for busy labs. Our cellSens™ software streamlines experiment setup and operation, providing you with more time to review results and make discoveries.
Optimized Performance, Trusted Results
Built on the stable IXplore IX85 platform and powered by Yokogawa spinning disk technology, the SpinSR system is optimized for high-performance super-resolution imaging. Our world-renowned optics add to this confidence, equipping your lab with a powerful set of tools to produce publication-ready images.
Your Sample in Focus
Capture high-precision, multipoint time-lapse images that are aligned and in focus with our TruFocus™ Z-drift compensator. With stable focus for long-term imaging and plate screening, you can be confident in the quality of your results.
LC3-EGFP expressing U2OS cells.
Range of Optimized Objectives
Evident’s expansive range of objectives can meet any research need, pushing your super-resolution system even further.
Simplified Immersion Imaging
The world’s first silicone gel pad objective (LUPLAPO25XS) combines all the quality and advantages of our silicone immersion objectives with the convenience and useability of a dry objective.
Left: confocal image
Right: TruSight SR image
Cultured Cos-7 cells, Tubulin Alexa Fluor 488. Easily switch from a confocal image to a higher resolution image using the LUPLAPO25XS objective lens with the TruSight SR super-resolution algorithm.
Consistent Quality
X Line series objectives redefine optical performance across applications with our revolutionary lens technology. X Line objectives offer simultaneously improved numerical aperture, flatness, and chromatic correction for bright, high-quality images throughout the field of view
Images acquired using an X Line UPLXAPO60XO objective lens.
Customized for Your Lab
Evident’s microscopy professionals will make sure you build the right IXplore IX85 SpinSR system for your lab’s unique needs. Customize every aspect of your spinning disk confocal super-resolution microscope, from the lasers to the eyepieces.
IXplore IX85 SpinSR (1-Deck System)
- High-quality confocal imaging system with a wider FOV
- Super-resolution imaging down to 120 nm resolution
IXplore IX85 SpinSR (2-Deck System)
- High-quality confocal imaging system
- Super-resolution imaging down to 120 nm resolution
- 2-deck frame structure for expanding your experiments
Specifications
| Laser | Laser lines | 405 nm: 50 mW, 445 nm: 75 mW, 488 nm: 100 mW, 514 nm: 40 mW, 561 nm: 100 mW, 640 nm: 100 mW |
|
| Combiner | Main combiner: 405 nm, 488 nm, 561 nm, 640 nm + 1 line (445 nm or 514 nm) Sub combiner: 445 nm, 514 nm |
||
| Light control | Direct modulation, on/off control and intensity modulation with individual laser lines | ||
| Motorized inverted microscope | IX85P1ZF | IX85P2ZF | |
| Scanner | Disk unit | Single SoRa disk or SoRa disk and 50 µm pinhole disk |
SoRa disk or 50 µm pinhole disk Maximum of 2 selectable disks |
| Camera port | 1 or 2 camera models* | ||
| Dichroic mirror | 3-position (motorized slider) | ||
| Filter wheel | 10-position (motorized wheel) | ||
| Adapter magnification | 1X / 2.8X / 4X | 1X / 3.2X | |
| Confocal imaging | Acquisition speed (max) | 5 ms/f | |
| Field number | 22 | 18.8 | |
| Super-resolution imaging | Acquisition speed (max) | 5 ms/f | |
| Field number | 7.9 / 5.5 | 5.9 | |
| Optical resolution** | SoRa disk: 110 nm 50 μm pinhole disk: 120 nm |
||
| Available objectives | LUPLAPO25XS (NA 0.85), UPLSAPO30XS (NA 1.05), UPLSAPO30XSIR (NA 1.05), UPLXAPO40X (NA 0.95), UAPON40XW340 (NA 1.15), UPLSAPO40XS (NA1.25), UAPON40XO340-2 (NA 1.35), UPLXAPO40XO (NA 1.40), UPLXAPO60XW (NA 1.2), UPLSAPO60XS2 (NA 1.35), PLAPON60XOSC2 (NA 1.40), UPLXAPO60XO (NA 1.42), UPLAPO60XOHR (NA 1.50), UPLSAPO100XS (NA 1.35), UPLXAPO100XO (NA 1.45), UPLAPO100XOHR (NA 1.50) | ||
| Imaging sensor | Third-party sCMOS camera | ||
| Imaging software | cellSens Dimension | Multi-dimensional acquisition and analysis | |
| Super-resolution imaging module (CS-S-OSR-VF) | |||
Related Products
IXplore IX85
The IXplore™ IX85 platform delivers an unmatched level of customizability, allowing you to design or build an intelligent, high-performance imaging system that meets your specific goals. And with an industry-leading field number (FN) of 26.5mm plus an array of advanced end-to-end imaging and workflow features, the IXplore™ IX85 means you can capture more than ever before while dramatically reducing your acquisition times.
IXplore IX85 Spin
The IXplore™ IX85 Spin system features a spinning disk confocal unit that enables fast 3D image acquisition, a large field of view, and prolonged cell viability in time-lapse experiments. Researchers can use it to perform rapid 3D confocal imaging with high resolution and contrast at greater depths for imaging into thicker samples. The spinning disk also helps to cut down on photobleaching and phototoxicity of samples upon excitation.
TruFocus™ Z-drift compensation system maintains focus for each frame
Precise 3D imaging with improved light collection using X Line™ objectives
IXplore IX85 SpinXL
See more and discover faster with an unparalleled 26.5 mm field of view (FOV).
- Capture more fast cellular dynamics in a single frame at imaging speeds up to 498 fps
- Precise, gentle live-cell imaging maintains sample viability
- Supports a wide range of applications and users with speed, precision, and flexibility
- Streamlines tasks throughout the lab with intelligent automation and easy workflows
- Evolves with your research via a wide wavelength range, tailored disk options, and expandable imaging modalities
- Powered by CrestOptics spinning disk technology and enhanced with the IXplore™ IX85 platform