IXplore IX85 SpinXL Spinning Disk Confocal Microscope System

See more and discover faster with the IXplore IX85 SpinXL spinning disk confocal microscope. With an unparalleled 26.5 mm field of view (FOV), you can capture cellular dynamics faster and screen large areas quickly. Precise, gentle live-cell imaging supports sample viability and image consistency.

Widen Your View into Cellular Dynamics: IXplore™ IX85 SpinXL Microscope

See more and discover faster with the IXplore IX85 SpinXL spinning disk confocal microscope. With an unparalleled 26.5 mm field of view (FOV), you can capture cellular dynamics faster and screen large areas quickly. Precise, gentle live-cell imaging supports sample viability and image consistency.

  • Streamlines tasks throughout the lab with intelligent automation and easy workflows
  • Supports many imaging applications and users by balancing speed, precision, and flexibility
  • Evolves with your research via a wide wavelength range, tailored disk options, and expandable imaging modalities
  • Powered by CrestOptics spinning disk technology and enhanced with the IXplore IX85 platform

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https://adobeassets.evidentscientific.com/content/dam/mis/ixplore-ix85-spin-xl/media/videos/Video-Project-13.mp4

3D epithelial breast cancer spheroids. DAPI (blue), pericentrin (yellow).

Expand Your View, Accelerate Your Insights

Optimized for fast, gentle live-cell imaging, the IXplore IX85 SpinXL rapidly acquires dynamic cellular events at high resolution. It reduces your imaging time for large sample sets so you can get all the data you need, faster.

Capture More in Every Frame

Quickly capture more cellular dynamics, such as calcium signaling, mitochondrial activity, or vesicle trafficking, in a single frame.

  • Unparalleled 26.5 mm FOV
  • Image at speeds up to 498 fps at full frame
  • Simultaneous dual-channel imaging

600 µm diameter HT-29 spheroid imaged with a 25X objective. DAPI (cyan), phalloidin (red).

A Wider Window into Cellular Dynamics

Whether you're working with large, thick samples or multi-well plates, the IXplore IX85 SpinXL speeds up the process and enables you to quickly acquire massive quantities of data.

With an unparalleled 26.5 mm field number (FN), you can capture large tissues using 30% fewer images than FN22, and 15% fewer images than FN25.*

*12×10 mm coronal rat brain section, 5 channels, 15 Z-layers.

Rat cerebellum. Hoechst (blue), Calbindin (green), MAP2 (orange), MBP (red).

See Further with Gentler NIR Imaging

Near-infrared wavelengths offer a range of benefits to your imaging:

  • Deeper tissue penetration due to reduced light scattering
  • Reduced autofluorescence and crosstalk in your samples
  • Gentler imaging for your live tissues, with reduced phototoxicity
  • Expanded multiplexing

https://adobeassets.evidentscientific.com/content/dam/mis/ixplore-ix85-spin-xl/media/videos/Colonmovie3.mp4

586 µm thick mouse colon.

Adaptable by Design. Ready for Every Researcher.

The IXplore IX85 SpinXL system can meet a wide range of applications, and its flexible, modular nature lets you expand as needs grow.

More Colors, More Information

Expansive multiplexing from 405 to 730 nm offers the flexibility to use the ideal wavelength for your fluorophores and image in up to seven channels, including in the NIR. Powered by the 89 North laser diode illuminator (LDI).

Neurons. DAPI (blue), ATP5 (yellow), vimentin (red), MAP2 (green).

Expandable to Dual Cameras

With dual-camera imaging, you can capture multiple fluorescence channels at the same time—so you get more data, faster. Start with one camera and easily upgrade when you're ready.

https://adobeassets.evidentscientific.com/content/dam/mis/ixplore-ix85-spin-xl/media/videos/expandable-to-dual-camera.mp4

Organoid. Phalloidin (magenta), E-cadherin (yellow).

Disk Boxes Tailored to Your Applications

Whether working with thick, dense, or other unique sample types, the IXplore IX85 SpinXL can meet your needs. Disk boxes are sealed to prevent dust or other contaminants from impacting image quality, and disk patterns can be switched as needed.

Standard disk

50 µm: 250 µm spacing

High-throughput disk

Spiral

Deep imaging disk

50 µm: 400 µm spacing

Workflows That Empower Efficiency

Drive efficiency with easy workflows for busy labs. Streamlined workflows in cellSens™ software offer quick setup and operation so you quickly get the best possible images from the system.

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Optimized Performance, Trusted Results

Powered by CrestOptics spinning disk technology and enhanced by the IXplore IX85 platform, the SpinXL is optimized for high-performance imaging. Our world-renowned optics add to this confidence, equipping your lab with a powerful toolset to produce publication-ready images.

Your Sample in Focus

Our TruFocus™ Z-drift compensator keeps your sample consistently in focus, enabling the capture of high-precision, multipoint time-lapse images. Whether you’re conducting long-term live cell imaging or high-throughput plate screening, be confident in the quality of your results.

https://adobeassets.evidentscientific.com/content/dam/mis/ixplore-ix85-spin-xl/media/videos/Media1.mp4

Cetuximab: Alexa Fluor 647 antibody drug (red). Natural Killer cells: ZsGreen (green). Detection of dead cells: DAPI (blue).

Microsecond Coordination

Our real-time controller works as the system’s conductor, ensuring each device is coordinated with microsecond accuracy.

  • Helps reduce the risk of sample damage
  • Delivers precise temporal data
  • Controls more devices via 16 TTL outputs and 16 analog outputs

Optimized Objectives

Evident’s expansive range of objectives can meet any research need, pushing your spinning disk system further.

Simplified Immersion Imaging

Our innovative multi-immersion objective with silicone gel pad technology (LUPLAPO25XS) combines all the quality and advantages of our silicone immersion objectives with the convenience and useability of a dry objective.

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Picture

XYZ image comparison between a LUPLAPO25XS silicone gel objective lens (left) and a UPLXAPO20X dry objective lens. Cleared HeLa* 1 cell spheroid (cyan: nuclei, magenta: microtubules). *To learn more about the origin of HeLa cells, visit henriettalacksfoundation.org.

Consistent Quality

X Line series objectives redefine optical performance across applications. Taking full advantage of the IXplore IX85 SpinXL’s 26.5 mm FOV, X Line objectives deliver exceptional correction of spherical and chromatic aberrations throughout a wide field of view. With high numerical apertures (NA) and outstanding transmission, they provide sharp, consistent images.

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Picture

Images acquired using an X Line UPLXAPO60XO objective lens.

Future-Proof Your Lab

Equip your lab with multiple imaging modalities to perform TIRF, FRAP, SMLM, and more on a single microscope.


Evident’s microscopy professionals will make sure you build the right system for your lab. Customize every aspect of your IXplore IX85 SpinXL microscope, from the lasers to the eyepieces.

IXplore IX85 SpinXL with Okolab incubation.

Specifications

Microscope frame IXplore IX85: IX85P1ZF, IX85P2ZF
Field of view (FOV) Up to 26.5 mm diagonal
Disk speed Up to 15,000 rpm
Disk patterns 50 µm pinhole / 250 µm spacing
50 µm pinhole / 400 µm spacing
50 µm slit
Custom disk patterns available
Resolution Lateral ~230 nm (1.4 NA)
Axial ~600 nm (1.4 NA)
Camera ports 2
Tested imaging speed Up to 498 fps for a full 26.5 FOV*
Widefield imaging Motorized bypass mode
Scanner spectral range Excitation: 400–750 nm; emission: 400–850 nm
Filter wheels and slider 3-position dichroic filter wheel
8-position emission filter wheel
4-position cleanup filter wheel
3-position slider for dual camera
Illumination Motorized iris to minimize sample exposure
Microlens array for uniform illumination
Light source 89 North laser diode illuminator (LDI); up to 7 channels
Available laser wavelengths 405 nm, 445 nm, 470 nm, 488 nm, 520 nm, 530 nm, 555 nm, 577 nm, 640 nm, 730 nm**
Laser and scanner control USB, TTL
Software cellSens Dimensions
Installation conditions Temperature: 23 ± 5 °C (73.4 ± 9 °F), humidity: up to 70% RH
Scanner weight 26 kg (57 lb)
Scanner dimensions (W × L × H) 357 × 606 × 225 mm (14 × 23.9 × 8.9 in.)
Scanner connector to IX85 Proprietary mount

*Captured using a Teledyne Kinetix camera with cellSens software.

**Not all wavelength combinations are available as standard.

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IXplore IX85

The IXplore™ IX85 platform delivers an unmatched level of customizability, allowing you to design or build an intelligent, high-performance imaging system that meets your specific goals. And with an industry-leading field number (FN) of 26.5mm plus an array of advanced end-to-end imaging and workflow features, the IXplore™ IX85 means you can capture more than ever before while dramatically reducing your acquisition times.

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Picture, Picture

IXplore IX85 Spin

The IXplore™ IX85 Spin system features a spinning disk confocal unit that enables fast 3D image acquisition, a large field of view, and prolonged cell viability in time-lapse experiments. Researchers can use it to perform rapid 3D confocal imaging with high resolution and contrast at greater depths for imaging into thicker samples. The spinning disk also helps to cut down on photobleaching and phototoxicity of samples upon excitation.

TruFocus™ Z-drift compensation system maintains focus for each frame

Precise 3D imaging with improved light collection using X Line™ objectives

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IXplore IX85 SpinSR

Capture ultra-small cellular dynamics in stunning super-resolution detail.

  • Discover more with high-speed imaging in resolution down to 120 nm
  • Designed for prolonged cell viability in long-term time-lapse experiments
  • Powered by Yokogawa spinning disk technology
  • Enhanced with the IXplore™ IX85 platform and Evident’s TruSight™ SR super-resolution algorithm

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