Fluorescence Microscopes

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A fluorescence microscope—also called a fluorescent microscope—is essential for capturing high-quality images of small specimens such as cells, tissues, and microorganisms. In epifluorescence microscopy, a specific type of fluorescence imaging, fluorophores are applied to the sample to emit light when excited by a high-intensity source. This technique enables you to generate detailed, multicolor images with high contrast and resolution.

Evident’s range of upright fluorescent microscopes use advanced epifluorescence microscopy, also known as reflected light fluorescence, to provide uniform and powerful illumination of the sample. Choose from semi and fully motorized upright fluorescent microscopes equipped with high-brightness LED light sources to streamline multicolor fluorescence imaging in research and clinical applications.

BX63

Automated Fluorescence Microscope

  • Fully motorized system allows automation of complex multidimensional experiments
  • Accurate motorized Z-drive
  • High stability due to fixed stage design

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BX53

Semi-Motorized Fluorescence Microscope

  • High-luminosity True Color LED illumination
  • Modular concept enables individual components to be motorized
  • Easily acquire multi-color fluorescence images

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CX43

Biological Microscope

  • Ergonomic design
  • Ideal for versatile applications
  • Long-lasting LED illumination*

*Optional single line LED light source for fluorescence (peak excitation wavelength of 470 nm only)

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FV5000

Confocal Laser Scanning Microscope

  • Extraordinary clarity, speed, and reliability driven by groundbreaking innovations
  • SilVIR™ detectors deliver photon-level quantitation, exceptional sensitivity, and ultra-high signal-to-noise
  • Unmatched dynamic range captures the full signal spectrum and prevents saturation
  • High-speed 2K resonant scanning and high-density 8K galvo scanning in one platform
  • FLUOVIEW Smart™ software simplifies operation with intuitive controls and AI-powered automation
  • TruResolution™ auto correction collar optimizes focus for over 20 objectives
  • Modular design supports up to 10 laser lines and future multiphoton upgrades
  • Laser Power Monitor (LPM) ensures stable illumination and reproducible results over time

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FV5000MPE

Multiphoton Laser Scanning Microscope

  • Compact fiber-pigtailed lasers enable deep, quantitative imaging in scattering tissue
  • One-, two-, or three-line simultaneous MPE laser excitation for millimeters deep imaging
  • SilVIR™, TruAI, and TruSight™ technologies deliver outstanding signal-to-noise and clarity
  • MPE-optimized objectives, TruResolution™ auto correction collar, and automated IR laser alignment maintain sharp focus
  • Available as an FV5000 system upgrade or a complete MPE system
  • Fully tunable laser configurations available for more advanced multiphoton applications

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FV4000

Confocal Laser Scanning Microscope

  • Game-changing dynamic range for imaging from the macro scale to subcellular structures
  • Multiplex up to six channels simultaneously with TruSpectral technology
  • Redesigned high-speed, high-resolution scanners for fixed and live cell imaging
  • Improved depth and photosensitivity with pioneering NIR capabilities and renowned optics
  • Peace of mind with the reliable, repeatable SilVIR detector
  • Industry leading * ten laser lines with a broader spectral range from 405 nm to 785 nm

*As of October 2023.

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IXplore IX85

The IXplore™ IX85 platform delivers an unmatched level of customizability, allowing you to design or build an intelligent, high-performance imaging system that meets your specific goals. And with an industry-leading field number (FN) of 26.5mm plus an array of advanced end-to-end imaging and workflow features, the IXplore™ IX85 means you can capture more than ever before while dramatically reducing your acquisition times.

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APX100

Benchtop Fluorescence Microscope

  • Easy-to-use, all-in-one microscope system
  • Publication-quality images in a few clicks
  • Fast, efficient data management

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CKX53

Compact Cell Culture Microscope

The CKX53 microscope eases the cell and tissue culture workflow, simplifying steps such as live cell observation, cell sampling and handling, image capture, and fluorescence observation. Its integrated phase contrast system, compact, ergonomic design, and stable performance enable simple, efficient cell observation. The universal sample holder and expandable stage accommodate a wide variety of cell culture container types and sizes.

  • Precentered phase contrast
  • Inversion contrast (IVC) technique provides clear three-dimensional views
  • Fluorescence with a 3-position slider
  • View multilayer tissue flasks up to 190 mm (7.5 in.) in height up thanks to the detachable condenser

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Upright Fluorescence Microscope FAQs

What is an upright fluorescence microscope?
An upright fluorescence microscope is a light microscope system that enables observation of the distribution of a single-molecule species based solely on the properties of fluorescence emission. Because it is an upright system, the sample is viewed from above. The sample must be prepared by treating it with fluorophores, which are chemical compounds that can re-emit absorbed light upon excitation. When you use an upright fluorescence microscope, the sample must also be sandwiched between a glass slide and a coverslip. Once excited by the light source, the sample re-emits light of a different wavelength that is then captured by the spectral emission filter to generate the image.
What is the difference between an upright and inverted fluorescence microscope?
The light path of a fluorescence microscope depends on a variety of factors, including the type of microscope. With an upright fluorescence microscope, the objectives are positioned above the specimen, whereas with an inverted fluorescence microscope, the objectives are beneath the stage and specimen. With an inverted microscope, the specimen can remain in its culture vessel or petri dish when the user observes it. With upright systems, samples are basically observed on a glass slide with a cover slip. This prevents the sample and objective from colliding. With both inverted and upright fluorescence microscopes, the specimen is treated with fluorophores.
When would you use an upright fluorescence microscope?
Upright fluorescence microscopes are the cost-effective workhorses of fluorescence imaging. For labs performing high-volume fluorescence observation, such as in life science research, upright epifluorescence microscope systems from Evident provide consistently high-quality bright, multicolor fluorescence images. Inverted epifluorescence microscopes are recommended when advanced imaging involving live-cell observation is required.

Fluorescence Microscopy Resource Videos

cellSens™ Imaging Software

This video shows you how to use cellSens imaging software with the DP74 digital microscope camera to improve your images acquired with upright fluorescence microscopes.

https://adobeassets.evidentscientific.com/content/dam/video/video/library/cellSens_DP74_Multiframe_Live_Noise_Reduction_MASTER.mp4

X Line™ High-Performance Objectives

Equip your upright fluorescence microscopes with X Line objectives to acquire accurate, bright multicolor fluorescence images with high image flatness and low chromatic aberration. See our novel polishing technique that enables us to manufacture these optical barrier-breaking objectives.

https://adobeassets.evidentscientific.com/content/dam/video/video/library/technical_introduction_en(2)_480.mp4