FLUOVIEW FV5000 Confocal Laser Scanning Microscope
Push the boundaries of microscopy further than ever before with the FLUOVIEW™ FV5000 confocal laser scanning microscope. Built on Evident's optical expertise and enhanced with advanced detection and scanning technologies, the FV5000 brings together quantitative imaging, speed, super-resolution, and reproducibility in one flexible platform.
SilVIR™ detectors deliver photon-level quantitation across a wide dynamic range, while SilVIR-RAY™ detectors extend this technology to fast, simultaneous multicolor super-resolution imaging. Combined with 2K resonant and 8K galvo scanning, the FV5000 captures everything from dynamic events to fine structural detail with confidence. Smart automation streamlines imaging workflows, while integrated performance monitoring supports consistent, reproducible results.
Built for the questions researchers ask today and ready for what comes next, the FV5000 turns complex imaging into confident discovery.
- Product Availability: This product is a replacement for the FV4000, FV3000, and earlier FV series systems.
- Product Status: For Research Use Only. Not for use in diagnostic procedures.
FLUOVIEW FV5000
No Detector Captures More. SilVIR-RAY™
Every confocal system promises great images. The FLUOVIEW™ FV5000 confocal and multiphoton laser scanning microscope delivers them with breakthrough SilVIR-RAY™ and SilVIR™ detector technology. Industry-leading linear dynamic range, true photon-resolving capability, and near-zero photon noise preserve every detail from acquisition to publication.
Every Color, Every Event, All at Once
Built from Evident’s legacy of innovation, FV5000 SilVIR-RAY detectors introduce true simultaneous multicolor super-resolution imaging, capturing bright and dim signals together and preserving spatial and temporal relationships in a single acquisition. Trusted, reproducible data reveal the dynamics without compromise. When the detector captures everything, the science speaks for itself.
The Detector That Changes Everything.
Powered by the SilVIR-RAY detector, the latest addition to the SilVIR detector family, the FV5000 brings fast, quantitative super-resolution imaging to demanding research in neuroscience, cell biology, drug discovery, cancer research, and developmental biology. The next-generation platform is designed to capture super-resolved, fully quantifiable data faster and easier than ever before:
- SilVIR-RAY enables true simultaneous multicolor super-resolution, with TruSight™ deconvolution achieving FWHM down to 80 nm lateral and 200 nm axial
- Unmatched dynamic range of 1 Gcps with high-SNR SilVIR detectors, trusted by leading research institutions
- High-density pixel scanners with 8K fast galvo and 2K high-speed resonant imaging
- FLUOVIEW Smart™ software interface and AI-powered workflows
- TruResolution™ automated correction collar for instant spherical aberration correction with over 20 of our standard objectives
- Modular, future-ready system design: integrate up to 10 laser lines and compact multiphoton upgrades
Try the FV5000 yourself—it will be the last laser scanning microscope you demonstrate.
Mouse cerebral cortex, layer V neurons expressing GFP, showing neuronal cell bodies and fine processes. SilVIR-RAY image with TruSight SR. Sample courtesy of: Drs. Shun Mitsui and Shigeo Okabe, Laboratory for Imaging Neural Dynamics, RIKEN Center for Brain Science (CBS).
Super-Resolution Imaging
Mouse brain section, hippocampal CA1 region. AAV-labeled neurons with GFP and VGAT immunofluorescence. 8K image acquired with a 25X oil immersion objective (1.00 NA, WD 1.0). Sample courtesy of: Dr. Megumu Takahashi and Prof. Hiroyuki Hioki, Department of Neuroanatomy, Juntendo University Graduate School of Medicine; and Drs. Hirotaka Asai and Shigeo Okabe, Laboratory for Imaging Neural Dynamics, RIKEN Center for Brain Science (CBS)
Brighter, Sharper, Faster
SilVIR-RAY super-resolution imaging makes every signal brighter—revealing sharper detail without dimming intensity or slowing you down. True simultaneous multicolor acquisition captures fast biological dynamics in real time while preserving quantitative image quality.
Two Scanners, One Workflow: High-Density Sampling and High-Speed Imaging
With the FV5000’s 2K resonant scanner for high-speed live imaging and 8K galvo scanner for high-resolution imaging across the full 20 mm FOV, you get bright, rapid workflows with nothing left in the dark.
The result: fewer compromises and faster time to data.
“More than beautiful images, SilVIR-RAY provides the image quality needed for quantitative analysis. Its wide dynamic range and unique objective lens can help improve both research confidence and analytical efficiency.”
Dr. Shigeo Okabe
Director, RIKEN Center for Brain Science (CBS), Tokyo
Every Channel. One Scan. Simultaneous Multicolor Super-Resolution.
SilVIR-RAY detectors capture up to four super-resolution channels simultaneously, without switching between channels. One scan preserves the temporal relationships between biological events, without the time offsets of sequential acquisition.
Wide dynamic range captures bright and dim signals together in every super-resolution channel.
High-resolution 3D volume rendering of apical dendrites from a Thy1-YFP H line mouse brain section, with dendritic spine heads clearly visualized. Objective: UPLAPO100XOHR (100X oil, NA 1.50). Sample courtesy of: Tetsushi Hoshida and Dr. Atsushi Miyawaki, RIKEN Center for Brain Science (CBS), Japan.
How SilVIR-RAY™ Works
1. Collects light and detail at once
The silicon photomultiplier (SiPM) array captures spatial detail while collecting light across a full Airy unit aperture.
2. Reassembles in real time
Our patented delay-line pixel reassignment realigns signals electronically as they are acquired, enabling fast, simultaneous multicolor super-resolution with reduced data load.
3. Reveals the finest detail
TruSight processing reveals detail down to 80 nm XY and 200 nm Z, while the SilVIR-RAY detector's wide dynamic range preserves information across bright and dim signals.
Learn More About the SilVIR-RAY Detector
Quantitation and Speed
MAP2 (green) and Hoechst (blue) in a cortical organoid at DIV 45 with cell line KOLF2.1J. Sample courtesy of: Declan J. Brennan, Nygaard Lab at UBC.
Quantitative Data You Can Defend
True photon-resolving capability delivers quantitative photon counts that remain comparable across experiments, users, and time points. The FV5000’s integrated Laser Power Monitor maintains identical illumination conditions from session to session. When reviewers ask whether a difference is biological or experimental, your quantitative photon counts provide the answer.
Absolute photon quantification, identical conditions—every time.
“The dynamic range of the detectors has allowed us to image a number of different labels that we were not able to previously accomplish without making compromises over what gets over- or under-exposed.”
Jonathan Epp, PhD
Department of Cell Biology and Anatomy, University of Calgary
SilVIR™ Next-Generation Detector Technology
Built on Evident’s patented silicon photomultiplier design, SilVIR technology captures every photon with exceptional sensitivity and the industry’s widest dynamic range. Low-noise electronics and a built-in Laser Power Monitor maintain illumination stability and ensure reproducible, quantitative results from faint signals to deep-tissue imaging.
Expect exceptional imaging from every level of signal. Clear high and ultra-low signals from the same sample with no compromise in quality. Histograms show discrete photon counts with quantifiable intensity and minimum background.
Top: Image saturation seen with a GaAsP-PMT detector (in red). Bottom: Same image captured with the SilVIR detector (no saturation).
Leave Saturated Images in the Past
With its expansive dynamic range, the SilVIR detector prevents signal clipping and minimizes time spent adjusting settings. Each capture delivers valid, unsaturated data ready for deconvolution, stitching, or spectral unmixing.
From single-photon events to intense fluorescence, SilVIR records the full signal spectrum in one acquisition. High dynamic range preserves faint details while preventing saturation of bright regions, reducing the need for reacquisition and ensuring consistent, quantitative image analysis.
Speed Without Noise Compromise
Stitched mouse brain slice cleared with SeeDB2. EYFP is expressed in cortical layer 5 pyramidal neurons in Thy1-YFP-H transgenic mice, acquired with a LUPLAPO25XO objective lens and resonant scanner. Sample courtesy of: Drs. Satoshi Fujimoto and Takeshi Imai, Graduate School of Medical Sciences, Kyushu University.
Acquire up to nine times faster than galvo with the same stunning clarity. Left: 43.5 minutes with 2K galvo Z-stack (galvo scan with no accumulation). Right: 4.6 minutes with 2K resonant Z-stack (resonant scan with 4x accumulation). Mouse brain cleared with SeeDB2. EYFP is expressed in cortical layer 5 pyramidal neurons in Thy1-YFP-H. Sample courtesy of: Drs. Satoshi Fujimoto and Takeshi Imai, Graduate School of Medical Sciences, Kyushu University.
Unmatched dynamic range combined with effortless Nyquist, big picture, and fine details. Galvo 8192 × 8192, zoom 0.9x, 0.8 a.u., LUPLAPO25XO objective lens, Ypet: 514 / 530-570, Z: 60-150 µm, Z step: 0.82 µm, MIP. Mouse brain slice cleared with SeeDB2. YPet is expressed in layer 2/3 pyramidal neurons (in utero electroporation). Sample courtesy of: Drs. Satoshi Fujimoto and Takeshi Imai, Graduate School of Medical Sciences, Kyushu University.
Capture Fast Responses
Thanks to the high SNR of the SilVIR detector, only minimal image averaging or accumulation is required to achieve high-quality results.
Live imaging of an acute olfactory bulb slice expressing GCaMP6f cleared with SeeDB-Live. Image acquired with a LUPLAPO25XS objective lens at 70 µm depth from the surface. Sample courtesy of: Drs. Shigenori Inagaki, Takeshi Imai, Graduate School of Medical Sciences, Kyushu University.
https://adobeassets.evidentscientific.com/content/dam/mis/fv5000/videos/product-page/70um-depth.mp4
Live imaging of an acute olfactory bulb slice expressing GCaMP6f cleared with SeeDB-Live. Image acquired with a LUPLAPO25XS objective lens at 120 µm depth from the surface. Sample courtesy of: Drs. Shigenori Inagaki, Takeshi Imai, Graduate School of Medical Sciences, Kyushu University.
https://adobeassets.evidentscientific.com/content/dam/mis/fv5000/videos/product-page/120um-depth.mp4
Live imaging of an acute olfactory bulb slice expressing GCaMP6f cleared with SeeDB-Live. Image acquired with a LUPLAPO25XS objective lens at 200 µm depth from the surface. Sample courtesy of: Drs. Shigenori Inagaki, Takeshi Imai, Graduate School of Medical Sciences, Kyushu University.
https://adobeassets.evidentscientific.com/content/dam/mis/fv5000/videos/product-page/200um-depth.mp4
Biology in Motion, from Cells to Embryos
See biology in motion, in detail. From FRAP to ablation, SilVIR quantitative detection and fast scanning allow you to capture weak signals with minimal averaging, delivering high SNR at real-time frame rates.
In living samples, precise stimulation enables repeatable, targeted perturbations, so you can track repair, migration, and signaling over time. A wide linear dynamic range helps prevent saturation, keeping time-lapse data analysis-ready.
Time-lapse sequence in a living zebrafish embryo showing the repair response after localized multiphoton ablation of microtubules (green). Sample courtesy of: Soraya Villaseca, PhD, Department of Physiology, Development and Neuroscience, University of Cambridge.
“The quality of imaging, I’ve never seen something similar before.”
Soraya Villaseca, PhD
Department of Physiology, Development and Neuroscience, University of Cambridge
Unmatched Simplicity
End-to-End Simplicity with FLUOVIEW Smart™ Software
Inspired by real researchers tackling the challenges of science, the FV5000 is transforming confocal imaging, making it smarter and faster.
- Smart Sample Search quickly locates your sample in XY and Z
- Smart Auto Laser Power Adjustment uses AI to optimize signal-to-noise across power setting
- Intuitive interface captures complex multidimensional images in just a few clicks
- Intelligent Shading Correction automatically creates seamless, high-quality stitched images
Note: FLUOVIEW Smart is available with FV5000 inverted configurations. FLUOVIEW Smart is not compatible with gantry, upright, or MPE configurations.
Smart Simplicity in Action
Automated Correction Collar Adjustment
The FV5000’s integrated TruResolution™ technology simplifies one of microscopy’s most tedious alignment tasks: objective collar adjustment. In a single click, the system automatically locates the optimal collar position for your sample, eliminating manual trial and error.
For thick specimens, TruResolution dynamically fine-tunes the collar during XYZ scans to maintain consistent image sharpness throughout the entire volume. Compatible with many standard objectives, it delivers uniform clarity across diverse samples and imaging conditions.
TruResolution technology automates correction collar adjustment. It fine-tunes objective settings to minimize spherical aberrations from cover glass and sample heterogeneity over depth, with broad objective compatibility.
Left: Without auto correction collar. Right: With auto correction collar. Automated correction collar adjustment delivers sharper, more detailed images
Image Segmentation Powered by Deep Learning
Go Beyond Conventional Thresholds
Traditional intensity-based thresholding can be slow, inconsistent, and highly sensitive to sample conditions.
TruAI image segmentation uses deep learning to recognize subtle patterns and faint signals that conventional methods miss, enabling accurate, reproducible segmentation of weakly labeled structures and complex tissues.
Clearly distinguish cells—and quickly find answers.
Spheroid imaging and analysis with TruAI. Left: Without TruAI. Right: With TruAI. TruAI segments and classifies cells (images on the right), even at high-penetration depths when the nuclei DAPI signal becomes weaker due to scattering.
Reliability and Flexibility
Engineered for Long-Term Precision, Built to Last
The FV5000 is engineered for long-term precision and adaptability, delivering the reliability and flexibility your science demands. Configure your system for today’s needs and expand effortlessly as your research evolves.
Add detectors, cameras, or lasers as workflows advance, or upgrade to multiphoton imaging with the MPE module, enabling single- and multiphoton acquisition as well as second and third harmonic generation. Smart hardware and software continuously monitor and optimize imaging performance, ensuring consistent, reproducible results. And with Evident’s global service and support network, every system is built to deliver lasting confidence and uptime.
Built for Reproducibility
The FV5000 maintains measurement precision through active system monitoring. The Laser Power Monitor (LPM) ensures consistent laser output across sessions, allowing different users to acquire images under identical conditions, even days or weeks apart. This stability supports the reproducibility required for quantitative and longitudinal studies.
To further safeguard performance, the Microscope Performance Monitor (MPM) automatically evaluates system sensitivity and imaging consistency. It detects deviations early, helping researchers maintain confidence in their results and ensuring every dataset reflects true experimental conditions.
More Colors and More Information
Capture more colors and extract more information from every image using the FV5000’s enhanced multiplexing capabilities. Updated TruSpectral™ technology, in combination with the high-sensitivity SilVIR detector, enables simultaneous acquisition of up to six channels, configurable with a selection of broadband and red-shifted detectors to accommodate a broader range of fluorochromes.
This configuration supports flexible experimental design, helping to reduce autofluorescence and photodamage during live-cell imaging. Modular laser combiners allow the integration of up to 10 laser lines, spanning wavelengths from 405 to 785 nm, to operate in parallel.
High-resolution five-channel confocal image of an embryo captured using DAPI and Alexa Fluor 488, 568, 647, and 750, revealing detailed structures across multiple fluorescence spectra.
Mouse brain slice expressing 7-color Tetbow cleared with SeeDB2 (in utero electroporation). Sample courtesy of: Drs. Satoshi Fujimoto and Takeshi Imai, Graduate School of Medical Sciences, Kyushu University.
Configurations
Built for Today. Designed for the Future
The FV5000 is built to grow with your science. Our next-generation SilVIR-RAY detector is a modular 1–4 channel upgrade and doubles as a standard confocal detector when super-resolution is not needed. The same SilVIR detector advantage that powers your super-resolution work powers every confocal and multiphoton experiment on the platform.
Expand When Your Science Demands It
Add detectors, cameras, or lasers as workflows advance, or upgrade to multiphoton imaging with the MPE module, enabling single- and multiphoton acquisition as well as second and third harmonic generation.
The FV5000 platform supports a full range of configurations to match your research, from IX85 inverted systems for high-speed live-cell imaging and upright frames for general imaging or electrophysiology to gantry setups for large or irregular specimens. For deeper imaging, the MPE configuration enables small-animal and thick-tissue studies, including large-frame and 3D organoid-optimized designs. Confocal and multiphoton modes can be combined in a single system, giving researchers unmatched versatility within one platform.
Confocal
Upright System
For glass slide sample imaging.
Upright System
For electrophysiology.
Gantry System
For in vivo observation that requires maximum space.
Inverted System
For observing tissue cultures, 3D cultures, and cell cultures (spheroids).
Multiphoton
Upright System
A large focus stroke accommodates a range of specimens, from tissue slices to live mice and other small animals.
Gantry System
The frame maintains a large working space below the objective, making it easier to position experiment equipment.
Inverted System
The frame supports observation of 3D cultures and multicellular clusters that are difficult to image using an upright frame.
Learn more about the FV5000MPE for mulitphoton applications.
World-Class Imaging Objectives
In addition to our award-winning X Line™ objectives, Evident offers an expansive range of A Line™ objectives that can meet any research need, pushing your confocal system even further.
Use our Objective Finder to locate the ideal objective for your application.
“The gel objective is my favorite thing ever. Image quality and acquisition speed, and also sensitivity, were all very impressive.”
Emma Steijvers, MSc, MPhil
AROS Lab, Department of Physiology, Development and Neuroscience, University of Cambridge
New Oil Immersion Objective
Our new long WD oil immersion objective lens allows you to see details deep in cleared samples.
- 25X / NA 1.0
- Working distance: 1 mm
- Immersion: oil
- High refractive index sample coverage: 1.45–1.56 (Rapiclear, BABB, etc.)
- APO chromatic aberration corrected
Whole mouse embryo cleared with Ethyl cinnamate, labeled with Alexa Fluor 405, 488, and 568. Captured in confocal mode (170 tiles in XY, 700 microns depth) with a 25X oil immersion lens (1 mm WD). Sample courtesy of: Dr. Emma Siragher, Hanna Group, Department of Physiology, Development and Neuroscience, University of Cambridge.
Driving the Future of High-End Imaging Research
The FV5000 combines precision engineering with intelligent automation to deliver reliable, reproducible results for every user—from core facilities to individual researchers.
- Core Facilities: Long-term stability with SilVIR detectors, automated calibration, and a modular, future-ready design that minimizes downtime and simplifies management.
- Research Leaders: Quantitative, publication-quality data through photon-level detection and automated workflows that accelerate discovery.
- Individual Users: Intuitive operation, rapid setup, and integrated performance monitoring ensure confident imaging from day one.
Service
Support and Service You Can Count On
When it comes to protecting your investment and the integrity of your research, your needs come first. We stand behind our products with a commitment to prompt service and technical support to help you achieve your goals.
Available in three convenient tiers—Maintenance, Protection, and Performance Plus—our FV5000 Service Plans* include priority support to help minimize downtime, regular scheduled maintenance to keep your equipment in peak condition, predictable repair costs to eliminate unplanned expenses, and direct, efficient solutions when you need them most.
*Regional variations in service offerings may apply.
FV5000 Service Plans
Specifications
FV5000 / FV5000MPE Combined Specifications
| SPECIFICATIONS | FV5000 / FV5000MPE | FV5000-RS / FV5000MPE-RS | |
| Scanner | Galvanometer scanner | 64 × 64 - 8192 × 8192 pixels, 0.2 µs/pixel – 1000 µs/pixels | |
| Resonant scanner | NA | 512 × 512 pixels, 1024 × 1024 pixels, 2048 × 2048 pixels | |
| Field number | 20 | ||
|
Spectral detector for confocal (SilVIR-B and SilVIR-R) |
Detector | SilVIR detector (cooled SiPM, broadband type; SilVIR-B/ redshift type; SilVIR-R) | |
| Maximum channels | Six channels1 | ||
| Spectral method | VPH, detectable wavelength range: 400–900 nm | ||
|
Super-resolution detector for confocal (SilVIR-RAY) |
Detector | SilVIR-RAY detector (array-based cooled SiPM) | |
| Maximum channels | Four channels1 | ||
| Spatial resolution |
120 nm lateral, 300 nm axial with TruSight SR2 algorithm 80 nm lateral3, 200 nm axial4 with TruSight deconvolution |
||
| CW laser for confocal | VIS laser | 405 nm, 445 nm, 488 nm, 514 nm, 561 nm, 594 nm, 640 nm | |
| NIR laser | 685 nm, 730 nm, 785 nm | ||
| Laser power monitor | Built in | ||
| Image | High dynamic range photon counting (1G cps), 16-bit | ||
| MPE-Specific Specifications | |||
| FV5000MPE/FV5000MPE-RS | |||
|
Non-descanned detector (SilVIR-B and SilVIR-R) |
Detector | SilVIR detector (cooled SiPM, broadband type; SilVIR-B/ redshift type; SilVIR-R) | |
| Maximum channels | Six channels1 | ||
| IR pulsed laser | Tunable laser |
One-laser system, dual-laser lines system, two-lasers system Excitation wavelength: 690–1300 nm Four-axis auto alignment, auto beam expander |
|
| Single-wavelength fiber-pigtailed laser | 920 nm, 1064 nm | ||
1 Combination restrictions may apply. Please contact your local Evident representative for details.
2 Design value based on the expected FWHM of 23 nm beads at 488 nm.
3 Measured lateral FWHM of 23 nm beads at 488 nm.
4 Measured axial FWHM of 100 nm beads at 488 nm.
Resources
Application Notes
How To
White Papers
Insights
Videos
HeLa cell spheroid labeled by DAPI (cyan, cell nuclei) and AlexaFluor790 (magenta, Ki-67)
HeLa cells labeled by MitoView 720
https://adobeassets.evidentscientific.com/content/dam/video/video/library/_7_1.mp4
TruFocus: Z-Drift Compensator
Silicone Oil Immersion Objectives: For Live Cell Imaging
