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Mapping Aortic Valve Cells Using the FLUOVIEW FV3000 Microscope

The valves of a healthy adult heart allow blood to flow to the lungs and throughout the body. However, the structure and function of the aortic valve are vulnerable to environmental stress. Understanding the cellular composition of the aortic valve is critical for the treatment of aortic valvular diseases, such as bicuspid aortic valve calcification and leaflet degradation.

By using the FLUOVIEW FV3000 confocal microscope, we successfully confirmed the contribution of Tie2 positive cells to the aortic valve identified by lineage tracing technology.

Easily Perform Macro-to-Micro Imaging of Murine Aortic Valve Cells

Thanks to the 1.25X macro imaging objective, we could easily and quickly acquire a large overview of an aortic valve slice with a single image. By simply dragging the mouse on the overview image, the FV3000 confocal microscope’s macro-to-micro function enabled us to quickly locate target cells and then observe the fine structures in a region of interest using a high-resolution objective lens.

https://adobeassets.evidentscientific.com/content/dam/video/image/application/fv-macro-micro-aorticvalve/movie_fv-macro-micro_v2.mp4

Video: Macro-to-micro overview of the expression pattern of Tie2 in the aortic valve of a mouse. Tie2 positive cells are labeled with EGFP (green), and non-endothelial cells are labeled with td Tomato (red).

Imaging equipment
Microscope: FV3000 confocal microscope with a motorized stage
Objective: 1.25X, 10X, and 40X objective (PLAPON1.25X, UPLSAPO10X2, and UPLSAPO40X2)

How the FV3000 Confocal Microscope Facilitated Our Experiment

Macro-to-Micro Observation

The FV3000 microscope’s macro-to-micro function provides a roadmap for data acquisition, enabling you to see data in context and easily locate regions of interest for higher resolution imaging.

Macro-to-Micro Observation

Extensive Lineup of Olympus Objectives

Olympus offers a wide range of objectives. The FV3000 optical light path supports objectives from 1.25X to 150X and acquires images of samples from large tissues to small organisms.

Extensive Lineup of Olympus Objectives

Acknowledgments
This application note was prepared with the help of Dr. Wenli Fan, State Key Laboratory of Pharmaceutical Biotechnology, Department of Cardiology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University.

References

  1. Feng, Q. et al. PDK1 regulates vascular remodeling and promotes epithelial-mesenchymal transition in cardiac development. Mol Cell Biol 30, 3711-3721, doi:10.1128/MCB.00420-10 (2010).
  2. Di, R., Yang, Z., Xu, P. & Xu, Y. Silencing PDK1 limits hypoxia-induced pulmonary arterial hypertension in mice via the Akt/p70S6K signaling pathway. Exp Ther Med 18, 699-704, doi:10.3892/etm.2019.7627 (2019).

FV5000

Confocal Laser Scanning Microscope

  • Extraordinary clarity, speed, and reliability driven by groundbreaking innovations
  • SilVIR™ detectors deliver photon-level quantitation, exceptional sensitivity, and ultra-high signal-to-noise
  • Unmatched dynamic range captures the full signal spectrum and prevents saturation
  • High-speed 2K resonant scanning and high-density 8K galvo scanning in one platform
  • FLUOVIEW Smart™ software simplifies operation with intuitive controls and AI-powered automation
  • TruResolution™ auto correction collar optimizes focus for over 20 objectives
  • Modular design supports up to 10 laser lines and future multiphoton upgrades
  • Laser Power Monitor (LPM) ensures stable illumination and reproducible results over time

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FV5000MPE

Multiphoton Laser Scanning Microscope

  • Compact fiber-pigtailed lasers enable deep, quantitative imaging in scattering tissue
  • One-, two-, or three-line simultaneous MPE laser excitation for millimeters deep imaging
  • SilVIR™, TruAI, and TruSight™ technologies deliver outstanding signal-to-noise and clarity
  • MPE-optimized objectives, TruResolution™ auto correction collar, and automated IR laser alignment maintain sharp focus
  • Available as an FV5000 system upgrade or a complete MPE system
  • Fully tunable laser configurations available for more advanced multiphoton applications

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FV4000

Confocal Laser Scanning Microscope

  • Game-changing dynamic range for imaging from the macro scale to subcellular structures
  • Multiplex up to six channels simultaneously with TruSpectral technology
  • Redesigned high-speed, high-resolution scanners for fixed and live cell imaging
  • Improved depth and photosensitivity with pioneering NIR capabilities and renowned optics
  • Peace of mind with the reliable, repeatable SilVIR detector
  • Industry leading * ten laser lines with a broader spectral range from 405 nm to 785 nm

*As of October 2023.

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Super Apochromat Objectives

PLAPON

For high-performance macro-observation, these apochromat objectives provide sharp, clear, flat images without color shift, achieving high transmission up to the near-infrared region of the spectrum. They perform well for fluorescence, brightfield, and Nomarksi DIC observations.

  • Displays flat images from high transmission factors up to the near-infrared region of the spectrum
  • Acquires sharp, clear images, without color shift, in fluorescence, brightfield and Nomarski DIC observations
  • Offers an unbeatable solution for every kind of digital imaging need for quality and performance

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