Imaging a super-risoluzione
Super-Resolution Microscopes Systems
IXplore SpinSR
Confocal Super Resolution for All Live Cell Samples
Achieve high-level super resolution 3D imaging down to 120 nm XY with the IXplore SpinSR confocal super resolution microscope. Its spinning disk system limits both phototoxicity and bleaching, providing imaging conditions conducive to live specimens. Easily switch between widefield, confocal, and super resolution as needed with a click of a button. The IXplore SpinSR can create accurate 3D reconstructions thanks to our high NA silicone immersion objective, which can minimize spherical aberration.
- Super resolution down to 120 nm XY resolution
- Prolonged cell viability in confocal time-lapse imaging due to less phototoxicity and bleaching
- Switch between widefield, confocal, and super resolution with one click
- Super resolution imaging with the world’s first plan apochromat objectives with a numerical aperture (NA) of 1.5*
* As of November 2018. According to Olympus research.
Abbelight SAFe Systems
Single Molecule Localization Microscopy
- Super Resolution at the molecular level, down to 10 nm
- Access a range of SMLM modalities and TIRF with one system
- Use as a standalone system, or add on to an existing widefield or confocal microscope
- Intuitive software and expert support to get meaningful results quickly
Related Applications
Olympus Super Resolution IXplore SpinSR
Fluorescence microscopes can identify specific proteins in vivo using fluorescent probes. The resolution of many of these microscopes is restricted by the diffraction limit to about 200 nm, making it impossible to observe fine structures. But with Olympus super resolution technology, you can acquire clear images with a resolution down to 120 nm in the horizontal direction.
How Olympus Super Resolution and Spinning Disk Technology Achieves Fast, Deep, and Reliable Live Cell Super Resolution Imaging
In the decades following the discovery of fluorescent proteins, live cell imaging has become an indispensable tool for the investigation of intracellular functions inside tissues and cells in the field of life science research. Today, various super resolution microscopy techniques are advancing fluorescence imaging even further. Super resolution imaging is attracting attention as a revolutionary tool in the life science field because it enables the observation of fine structures that are less than 200 nm (nm=10-9 m) in size, an imaging performance that is unattainable using a conventional optical microscope. However, applying super resolution microscopy techniques to live cell imaging is limited because of time resolution and phototoxicity issues. In addition, the reliability (quantitative performance) of imaging data is also a significant concern because image processing including estimation or spatial frequency shift can form artifacts that corrupt results.
How the TruSight™ SR Algorithm Achieves Super-Resolution Imaging in Confocal Spinning Disk Microscopy
Evident’s TruSight SR algorithm is a novel super-resolution technology evolved from Olympus Super Resolution (OSR)1 and integrated into the IXplore™ IX85 SpinSR confocal spinning disk super-resolution microscope. This technology enhances confocal microscopy by optimizing the pinholes and employing an optical theory-based processing engine to achieve super-resolution imaging.
TruSight SR addresses OSR's limitations in low signal-to-noise ratio (SNR) images by incorporating adaptive filter strength and three-dimensional (3D) processing capabilities. This advancement supports the enhancement of image quality in low SNR conditions, such as live-cell imaging with dim fluorescence signals. By restoring natural super-resolution images and preserving brightness values, TruSight SR enables consistent image data that support quantitative analysis across diverse applications.
Related Categories
Super-Resolution Microscopy FAQs
What is super-resolution microscopy used for?
How can I improve my image resolution with Evident's super-resolution microscope solutions?
Since an objective’s resolution is determined by its emission wavelength and numerical aperture (NA), choosing a high NA objective lens is an effective way to improve imaging resolution. Evident X Line objectives and our A Line HR objective lens have higher numerical apertures.
To improve the resolution during 3D super resolution of live cells, minimizing spherical aberration due to refractive index mismatch is important. Our A Line silicone oil immersion objectives provide a close refractive index match to that of live cells, enabling brighter and higher resolution 3D imaging with minimal spherical aberration.