cellSens Microscope Imaging Software

Providing intuitive operations and a seamless workflow, cellSens software’s user interface is customizable so you control the layout. Offered in a range of packages, cellSens software provides a variety of features optimized for your specific imaging needs. Its Graphic Experiment Manager and Well Navigator features facilitate 5D image acquisition. Achieve improved resolution through TruSight™ deconvolution and share your images using Conference Mode.

The cellSens software package is not for clinical diagnostic use.

Software Downloads:

cellSens Software

cellSens Imaging Software

Intuitive Operation. Seamless Workflow.

The Evident cellSens platform gives you full control over the display and placement of icons, toolbars, and controls, enabling the software to grow and adapt to meet your evolving research needs.

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cellSens Packages

cellSens Entry

cellSens Entry is the ideal stepping stone for researchers wanting to move into digital image acquisition and documentation, providing all the tools needed for simple image acquisition.

cellSens Standard

The cellSens Standard takes acquisition beyond a single image, with advanced image capture processes (e.g. time lapse) and control of motorized and encoded microscope components.

cellSens Dimension

cellSens Dimension is the most versatile cellSens software package, featuring fully automated image acquisition, powerful analysis tools, and a wide range of additional benefits.

5D Experiment Acquisition

Acquire images in five dimensions using tools like the Graphic Experiment Manager (GEM) and Well Navigator that help you visualize your data acquisition in a user-friendly way.

Graphic Experiment Manager (GEM)

Dimension

The GEM is a flexible drag-and-drop interface that enables you to build simple or complex experiments within cellSens software. Combine actions within specialized frames to dictate the order and priority of automation and interact with the system during long-term, time-lapse acquisitions without terminating the experiment. To increase efficiency, you can define macro functions, such as executing deconvolution processing in the GEM.

Process Manager

Dimension + Standard

The Process Manager makes it easy to capture multichannel and time-lapse images with just a few clicks. Z-stack imaging is also possible when using a cellSens Dimension license.

Dimension + Multiposition

Standard + Multiposition

Use the optional Multiposition solution to automatically capture multipoint and large-area images when using a motorized stage. You can also navigate around your sample by simply clicking on a point in an image with the Stage Navigation tool.

Dimension + Multiposition + Well Plate Navigator

The Well Plate Navigator automatically scans and acquires images from standard and customized well plate formats. All acquired images, sample positions, and user comments can be saved into a structured database for rapid centralized access. Apply unique multidimensional acquisition settings to a single well or multiple selected wells in one step, so you can execute multiple experiments within a well plate in support of complex experiments.

Database

Dimension + Database Core or Database Client

Standard + Database Core or Database Client

The Database Core solution enables you to create user-defined databases with full access control, which can be shared across a network. An interactive query tool makes it easy to find the desired images, associated image properties, user comments, and all related files, like spreadsheets, with automatic preview of the found images. With the Database Client solution, you can conveniently read and write to the shared database from many different stations.

Dimension + Multiposition + Well Navigator +

Database Core or Database Client

When used with the Well Navigator solution, the Database solution makes viewing and analyzing well plate images with a large amount of data more efficient. Simply click on icons for image information, such as the date, file name, or well plate number, and any selection of captured images can be viewed for further analysis. Together, these tools enable you to view captured images and continuously analyze selected images (using Batch Macro commands) using the well plate interface.

Real-Time Panoramic Imaging

Dimension

Standard + Manual Process

Create stitched images in real time with the Manual Process solution. Manual Process Control makes it simple to move around your sample using a manual stage while the software records and stitches the images in real time, providing a cost-effective alternative to whole slide imaging.

Dimension + Multiposition

Standard + Multiposition

With cellSens Dimension, and a motorized stage, large-area image acquisition is fully automated with the optional Multiposition solution. When combined with a motorized Z or focus maintenance device, like our TruFocus™ technology, this function can correct for the effects of sample distortion and tilting.

https://adobeassets.evidentscientific.com/content/dam/video/image/landing-directory/cellsen/cellSens_mia_e_480.mp4

EFI

Dimension

Standard + Manual Process

Create a single in-focus image from successive image planes as you turn the focus knob using the Extended Focus Imaging (EFI) function. A motorized focus drive fully automates EFI acquisition. EFI composite images can also be created directly from previously captured Z-stacks.

HDR Acquisition

Dimension

By automatically capturing many images at different exposures, the HDR function creates a final image with a higher dynamic range than could be achieved on a single exposure. Low-intensity signals are clearly visible without overexposing the bright areas of the sample.

Reveal the true data in your images with TruSight deconvolution and other image processing techniques. Easily share your results with others using Conference Mode or drag and drop data into preconfigured reports.

Powerful TruSight™ Deconvolution

Dimension

cellSens Dimension includes live 2D deblurring for image preview and acquisition to enable better focusing on thick specimens.

Dimension + CI Deconvolution

The Constrained Iterative Deconvolution solution has the latest algorithms for improved resolution, contrast, and dynamic range with industry-leading speed through GPU processing. Algorithms designed specifically for use with FLUOVIEW™ and Olympus Super Resolution (OSR) images provide clearer, sharper images from high-end systems utilizing ultrafast frame rates and high sensitivities.

Cell line: Human cervical cancer cell line (HeLa)^*1
Immunostaining: Hec1 staining (green, Alexa Fluor-488), a-tubulin staining (red, Alexa Fluor-568), DAPI staining (blue)
Mitotic HeLa cell derived from human cervical cancer. Mitotic spindle and kinetochores are stained with anti-a-tubulin (red) and anti-Hec1 (green) antibodies, respectively. Chromosomes interact with microtubules constituting mitotic spindle via kinetochores, protein structure assembled on centromere region of chromosomes.
Image data courtesy of: Department of Molecular Oncology, Institute of Development, Aging, and Cancer, Tohoku University Masanori, Ikeda and Kozo Tanaka

*To learn more about the origin of HeLa cells, visit henriettalacksfoundation.org.

Focus on What's Important

Dimension

Objectively determine the best focus from multidimensional images, including Z-stack and time-lapses, using the Best Focus Extraction tool. This tool is effective in creating T-series images with the best focus possible.

Visualize three-dimensional data in a single plane using maximum intensity projections, which combine Z-series planes into a single image with the brightest pixels all in view.

Real-Time Collaboration

Dimension

Standard

Use Conference Mode to fill the screen with live or static images for presentation and collaboration. Graphic annotation tools are available at your fingertips for image markup without the need to exit Conference Mode, improving workflow efficiency and saving time.

Dimension + NetCam

Standard + NetCam

Using standard TCP/IP protocols, the cellSens NetCam Solution facilitates the transfer of live and stored images throughout a network for teaching, mentoring, or supervision. Even when outside of the laboratory, colleagues or supervisors can monitor the work from any point on the network, improving the efficiency of the laboratory.

https://adobeassets.evidentscientific.com/content/dam/video/video/library/04-ConferenceMode_E_SD_Revised.mp4

A Different Perspective

Dimension

Standard

Display images side-by-side for accurate comparison with simultaneous zooming and movement for faster image processing.

Dimension

Tile View mode enables you to view multidimensional datasets all at once, making fine movements or events easier to identify.

Simple Data Reporting

Dimension

A convenient Reporting tool combines images and measurement metadata into a report template with easy drag-and-drop operation. These Microsoft Word^*2 reports enable you to quickly and easily collaborate with colleagues and communicate results.

_{*2 Requires Microsoft Word version 2010 or later}

Spectral Unmixing

Dimension

With the linear unmixing algorithm in cellSens Dimension, fluorochromes that overlap in their emission spectra—such as GFP and YFP—can be readily separated to produce crosstalk-free fluorescence images. This linear unmixing tool can also separate autofluorescence-related background signals.

Powerful Analysis Tools

Dynamically work with your images to extract the most data for reliable experimental results. The software’s TruAI deep-learning technology offers improved segmentation analysis. Use the Macro Manager to automate entire workflows all the way through image analysis and saving.

Green: You can see that the detection accuracy is low due to unevenness of the GFP label.

Blue: Detecting the nuclei with high accuracy despite scratches and dust on vessel.

TruAI Deep Learning

Dimension + Deep Learning

Standard + Deep Learning

Experience the benefits of cellSens software’s TruAI deep-learning technology to improve your image analysis. From automatic segmentation of complex morphologies without hand labeling to segmentation of cells or organelles using a simple transmitted light image, deep-learning technology offers improved speed and efficiency without the phototoxicity of fluorescence.

Explore Application Examples

Learn More About TruAI

Object Count and Measure

Dimension

Standard + Count and Measure

Efficient and precise threshold-based object detection for automated nuclei counting and classification are available. Conveniently export your results to Microsoft Excel for additional analysis.

Dimension + Count and Measure

Standard + Count and Measure

Expand on the extensive manual measurements already available in cellSens software with the Count & Measure module. Easily perform automatic object measurement and classification in an interactive interface where recognized objects are always linked with their measurements.

Dimension + Count and Measure + Deep Learning

Standard + Count and Measure + Deep Learning

Improve your count and measurement by detecting objects with deep learning.

https://adobeassets.evidentscientific.com/content/dam/video/video/library/cellSens_tracking-web2(2)_406.mp4

Live-Cell Solutions

Dimension

Standard + Confluency Checker

Our specialized cell identification algorithm enables you to measure cell count and confluency on phase contrast images, including data averaging and total cell count estimation. A cell growth curve can be output by measuring along with time series.

Dimension + Count and Measure + Tracking

cellSens software's Object Tracking solution provides a powerful and intuitive tool to quantify dynamic processes such as cell movement and division. Moving objects can be automatically detected, tracked, and analyzed over time.

Intensity Measurement

Dimension

Graphically depict intensity and ratio values defined by regions of interest (ROIs) and adjust ROI placement to compensate for cell movement. Convert intensity variations to hue and brightness using intensity modulated display (IMD) to visually enhance the fine structures often found within ratio or FRET images.

Dimension + Ratio/ FRET or Life Science Analysis

The Ratio/FRET solution is used to display and analyze real-time ratiometric imaging data. FRET analysis of both sensitized emission and acceptor photobleaching is supported in this user-friendly workflow.

Dimension + Life Science Analysis

The Photo-Manipulation Solution can be used for curve-fitting analysis of FRAP images.

Simple Macro Experiments

Dimension

Save time and clicks by using the macro manager to automate typical acquisition and data analysis workflows. Batch Macro commands can be applied to multiple images simultaneously and can reduce the time required to complete complex image acquisition and analysis.

Customizable User Interface

Choose a recommended layout for image acquisition and analysis or create your own using the My Functions tool set.

Intuitive at All Skill Levels

Dimension

Standard

Novice and expert users will simultaneously benefit from the Simple Layout, an interface designed for a clinical research workflow.

Acquire, annotate, share, and save your images using the intuitive Smooth Control tool window.
Built-in measurement tools display only when required, reducing software clutter and minimizing distraction.

More complicated experiments benefit from premade layouts where all common functions are integrated into tabs, combining related options and settings. Layout tabs make it easy to select functions according to the workflow. For example, camera control features are displayed under the Acquisition tab, but are hidden when you switch to the Processing tab in the next stage.

https://adobeassets.evidentscientific.com/content/dam/video/video/library/cellSens_SimpleLayout_e(2)_404.mp4

Build Your Own Layout

Dimension

Standard

Tools and windows can be organized to suit the job at hand, optimizing the layout’s functionality. This enables you to spend less time training and more time imaging.

You can create and save custom toolbars for frequently used functions and save them to the My Functions window, where they can be used to improve efficiency when working with your most commonly used tools.

Minimize Monitor Glow

Dimension

Standard

The Dark Application Skin reduces computer monitor-generated ambient light, helping you adapt to darkened environments, like those required when imaging samples with fluorescence. Icon contrast remains high for easy recognition and quick selection.

Specifications

cellSens Solutions Software Modules	y = included O = optional	Entry	Standard	Dimension
Manual Process	Easily create high-resolution composite images (Instant MIA) by simply moving the manual stage. You can also acquire a focused image (EFI) over the entire surface by manually shifting the Z direction.	o	o	y
Encoded Device	Encoded devices (objectives, light intensity, etc.) make it easy to recall settings.	o	y	y
Interactive Measurement	Draw a polyline, rectangle, or circle on top of your image to obtain exportable measurement data. Measurement results can be exported to Excel.	o	y	y
Database Client	Access to the database created with the Database Core option.	o	o	o
Database Core	Make data management and browsing more efficient by creating a database that can easily search and sort acquired images based on data, such as imaging conditions and acquisition date.		o	o
Confluency Checker	Determine the confluency of unstained live cells in culture dishes through quantitative measurements for reliable data.		o	y
Multiposition	Multipoint and stitched images can be acquired using the motorized stage. When combined with the motorized Z, a focus map can be created from multiple points of focus, and you can obtain stitched images with little focus deviation by removing sample tilt and distortion.		o	o
Count & Measure	Define the morphology of an object, and the software will identify all similar objects and present segmentation analysis results in a chart.		o	o
NetCam	Facilitates the transfer of live and stored images through a network for teaching, mentoring, or supervision.		o	o
Deep Learning	Efficient segmentation analysis powered by deep learning enables challenging target detection, such as label-free nucleus detection.		o	o
Well Plate Navigator*¹	Easily set the capture settings for each well. The well position and name can be tagged to images, making data management easier and well plate screening more efficient.			o
CI Deconvolution	Access to GPU-based deconvolution as well as popular and custom TruSight deconvolution algorithms to improve the sharpness, contrast, and dynamic range of reconstructed images.			o
Ratio/FRET	Obtain ratio measurements from your images as they’re being acquired.			o
Tracking*²	Measure and analyze the luminance and speed of individual cells that move and divide over time.			o
Life Science Analysis	FRAP/FRET analysis can be performed on the acquired image.			o
Photo Manipulation	Enables cell frap module control and FRAP analysis.			o
Laser Control	Enables NI USB-6343 BNC to control external devices.			o
Automated Correction Collar (ACC)	Operating automated correction collar.		o	o
Super Resolution for cellSens*³	Renewed super resolution (online and offline)			o

^{*1 Requires Multiposition option}

^{*2 Requires Count & Measure option}

^{*3 Also for Desktop}

cellSens Functions	✓ = included - = not included	Dimension	Standard	Entry
Layout	User experience customization	✓	✓	✓
View	Overlay multiple images	✓	✓	-
	Document groups for side-by-side image comparison	✓	✓	✓
	Movie playback	✓	✓	✓
	Tile view (multiple images in a single data set shown side-by-side)	✓	✓	✓
	Slice view for orthogonal plane viewing of 3D or time-lapse data sets	✓	-	-
	Voxel viewer for isosurface and volumetric rendering of 3D and 4D data sets	✓	-	-
Image Acquisition	Snap/movie acquisition	✓	✓	✓
	Time-lapse at specified interval	✓	✓	-
	Automated multiwavelength	✓	✓	-
	Z-stack	✓	-	-
	Multidimensional (XYZT and wavelength)	✓	-	-
	Graphical Experiment Manager	✓	-	-
	Manual panoramic imaging (Instant MIA and Manual MIA)	✓	Manual Process	Manual Process
	Multiposition visitation and stage navigator	Multiposition	Multiposition	-
	Automated panoramic imaging (auto MIA, requires motorized stage)	Multiposition	Multiposition	-
	Instantly create EFI image (manual or motorized Z)	✓	Manual Process	Manual Process
	Simultaneous multicolor Imaging (requires two identical cameras** or image splitter)	✓	-	-
	Live deblurring	✓	-	-
	High dynamic range imaging (HDRI)	✓	-	-
	Multiwell plate acquisition	Well plate navigator and Multiposition	-	-
	Automated correction collar (ACC)	ACC	ACC	-
	Super-resolution filter with renewed algorithm	✓	-	-
Image Processing	Geometry/combine/filter processing	✓	✓	-
	Fluorescence unmixing	✓	-	-
	Brightfield unmixing	Count & Measure	-	-
	Deblurring (No/Nearest Neighbor, Wiener Filter)	✓	-	-
	Kymograph	✓	-	-
	2D deconvolution	✓	-	-
	3D deconvolution （constrained iterative deconvolution with GPU process）	CI Deconvolution	-	-
Deep Learning (TruAI)	Training of neural networks	Deep Learning	Deep Learning	-
Deep Learning (TruAI)	Inference using trained neural networks (offline/online)	Deep Learning or Count & Measure	Deep Learning or Count & Measure	-
Image Analysis	Phase analysis	✓	-	-
	Object measurements and classification	Count & Measure	Count & Measure	-
	Interactive 2D measurements	✓	✓	✓*
	Intensity plot over time/z	✓	-	-
	Colocalization	✓	-	-
	Object counting (manual)	✓	✓	-
	Object tracking	Tracking and Count & Measure	-	-
	Online ratio and kinetics	Ratio/FRET	-	-
	Ratio analysis (offline)	✓	-	-
	FRET analysis	Ratio/FRET or Life Science Analysis	-	-
	FRAP analysis	Photo Manipulation or Life Science Analysis	-	-
	Cell count and confluency measurements	✓	Confluency Checker	-
Documentation and Collaboration	Automatically compose MS Word reports	✓	-	-
	Database image and data management solution for microscopy	Database Core	Database Core	-
	Open database and load records/documents from database	Database Client	Database Client	Database Client
Remoting	Remote live image viewing	NetCam	NetCam	-

^{* Three-point angle, four-point angle, arbitrary line, closed polygon, polyline, and perpendicular line only. Interactive measurement option is needed to add other measurement tools and make exporting Excel spreadsheets possible.}

^{**Supported cameras: iXon Ultra 897, Zyla 5.5 (USB 3.0), Zyla 4.2 (USB 3.0/CamLink), Neo, iXon Ultra 888, ImagEM X2, ORCA-Flash 4.0 (V3), Prime 95B, Prime BSI, Prime BSI Express, Sona4.2B-11, Kinetix, Kinetix 22, ORCA-Fusion, ORCA-Fusion BT, ORCA-QUEST.}

Products with Confirmed Functionality		✓ = included - = not included	Dimension	Standard	Entry
Olympus/Evident	Camera	DP22, DP23, DP23M, DP27, DP28, DP74, DP75, DP80, XM10, UC90, LC20, LC30, LC35, SC50, SC180	✓	✓	✓
	Micoscope	BX43, BX53, BX63, BX61, BX61WI, IX83, IX85, IX73, IX81, SZX16A	✓	✓	-
	Micoscope	IX81-ZDC, IX81-ZDC2	✓	-	-
	Peripherals	BX-DSU, IX3-DSU, IX3-ZDC, IX3-ZDC2, IX2-DSU, IX2-ZDC, IX2ZDC2, U-CBF, cellTIRF (multiline, single line), USB-ODB converter, Real Time Controller (U-RTCE)	✓	-	-
	Light Source	U-LGPS	✓	✓	-
Hamamatsu	Camera	ImagEMX2, ORCA-Flash 4.0 V3, ORCA-Flash 4.0 LT PLUS, ORCA-Flash 4.0 LT3, ORCA-Fusion, ORCA-Fusion BT, ORCA-QUEST, ORCA-Halo*	✓	-	-
	Camera	ORCA-spark	✓	✓	-
	Image Splitter	W-View Gemini	✓	-	-
Q-Imaging	Camera	Retiga 6000	✓	-	-
Photometrics	Camera	Prime (PCI-Express), Prime 95B, Prime BSI, Prime BSI Express, Moment, Kinetix, Kinetix22	✓	-	-
Photometrics	Image Splitter	Dual View DV2 / QuadView QV2	✓	-	-
Andor	Camera	iXon Ultra 897, iXon Ultra 888, iXon Life 888, iXon Life 897, Sona4.2B-11,Zyla4.2/Zyla4.2 PLUS (Camera-link,USB3.0), Zyla5.5 (Camera-link 10tap,USB3.0), ZL41 Cell 4.2 (Camera-link,USB3.0), Neo5.5	✓	-	-
Vincent Associates	Shutter	Uniblitz shutter (VCM-D1, VMM-D1, VMM-D3)	✓	✓	-
CoolLED	Light Source	pE-1, pE-2, pE800, pE-4000, pE-400 max	✓	-	-
CoolLED	Light Source	pE-300white, pE-300ultra, pE-340fura	✓	✓	-
Excelitas	Light Source	X-Cite120LED, X-Cite XYLIS, X-Cite TURBO, X-Cite NOVEM	✓	-	-
Lumencor	Light Source	SOLA SEII, SEII 365, Spectra X	✓	-	-
Sutter	Shutter, FW	Lambda 10-3/10-B	✓
Prior	Motorized XY Stage	ProScan III, Optiscan III	Multiposition	-	-
	Shutter, FW, Z-drive	ProScan (I, II, III) , Optiscan III	✓	-	-
	Piezo Z (Control via Real Time Controller)	NanoScanZ NZ100	✓	-	-
Ludl	Motorized XY Stage	Mac 6000	Multiposition	-	-
Ludl	Shutter, FW, Z-drive	Mac 6000	✓	-	-
Märzhäuser	Motorized XY Stage	Tango, Pilot Stage	Multiposition	-	-
Märzhäuser	Z-drive Controller	Tango	✓	-	-
Physik Instrumente	Piezo Z (Control via Real Time Controller)	PIFOC P-721	✓	-	-
Applied Scientific Instrumetation	Motorized XY Stage	MS-2000	Multiposition	-	-
Applied Scientific Instrumetation	Z-drive Controller	MS-2000	✓	-	-
National Instruments	Digital TTL Device	NI USB-6501	✓	-	-
National Instruments	Digital TTL Device	NI USB-6343 BNC	Laser Control	-	-
Yokogawa	CSU	CSU-X1, CSU-W1	✓	-	-
Yokogawa	Magnification Changer	IX SPIN-SR	✓	-	-
CrestOptics	CSU	X-Light V3	✓	-	-

^{For details on Windows OS compatibility, please contact your Evident sales representative.}

^{*Not in combination with other Hamamatsu cameras}

Compatible image formats

Read and write

JPEG, JPEG2000, TIFF, BMP, AVI, PNG, VSI, PSD (Adobe Photoshop), Big TIFF, OIR

Read only

GIF, OIF/OIB (FLUOVIEW format), Cell, STK (MetaMorph), MRC (Medical Research Council)

System Requirements

Microsoft Windows 10 Professional (64-bit) (22H2), Microsoft Windows 11 Pro (64-bit)(24H2)

OS Language

English, Simplified Chinese, Japanese, German and Italian (Entry and Standard)

CPU

Intel Core i7, Intel Core i9, Intel Xeon; Recommended for high-speed image acquisition: at least QuadCore

RAM

8 GB for general applications, 16 GB or more is recommended for high-speed image acquisition, 32 GB or more is recommended for deep learning（For DP23/DP28/DP23M, dual memory is recommended for high frame rate imaging)

HDD

Up to 7 GB for installation

Recommended for high-speed image acquisition: solid state drive (SSD)

Web Browser

Recommended: Microsoft Edge

Software Version Update

A version update is available for the next version following the version written on the license card (excludes updating sub-minor versions). An update that spans two or more major or minor versions requires an update license, which provides access to the latest version of cellSens software after the above period.

Check the latest version for your software: License Information