TruFocus Technology | Olympus LS

TruFocus Technology Stable Time-Lapse Imaging

The TruFocus system makes multiposition experiments and long-term, time-lapse imaging more robust and reliable. Learn more.

The TruFocus system makes multiposition experiments and long-term, time-lapse imaging more robust and reliable.

An accurate beam maintains the focus position, compensating for thermal drift caused by changes in room temperature or drug delivery during experiments
TruFocus Red system maintains the focus position during near-infrared (NIR) imaging when using dyes like Cy7

Focus find mode allows you to easily find the focus position of your sample
Single-shot autofocus (AF) mode allows you to perform multi-focus multi-position experiments for efficient 3D and 4D imaging
Continuous AF mode enables to focus your sample in real time for high resolution, long term time-lapse imaging
Compatible with plastic and glass vessels

Application note: Neurovascular Structure

Application note: Neurovascular Structure

Application note: Neurovascular Structure

Application note: Neurovascular Structure

Utilize the Olympus real time controller for physiologically relevant data with minimal cell disturbance
Maintain cell viability while imaging with various environmental control options
Maintain focus accurately and reliably in time-lapse experiments with TruFocus
Discover the real shape of your cells with Olympus silicone immersion optics

Excellent simultaneous multi-color TIRF for investigation of membrane dynamics and single molecule detection
Exact colocalization of up to four markers thanks to individual penetration depth control
Take advantage of Olympus’ remarkable TIRF objective with the world's highest NA of 1.7* (*As of July 25, 2017. According to Olympus research)
Intuitive set-up of complex experiments with Graphical Experiment Manager (GEM)

Rapid and high-resolution confocal imaging with a spinning disk system
3D confocal time-lapse imaging of live cells with less phototoxicity and bleaching
Precise 3D imaging with improved light collection using silicone oil immersion objectives
Upgrade to the IXplore SpinSR super resolution system depending on your research progress and/or budget

Super resolution down to 120 nm XY resolution
Prolonged cell viability in confocal time-lapse imaging due to less phototoxicity and bleaching
Switch between widefield, confocal and super-resolution with one click
Accurate 3D reconstruction with Olympus silicone oil immersion objectives

Game-changing dynamic range for imaging from the macro scale to subcellular structures
Multiplex up to six channels simultaneously with TruSpectral technology
Redesigned high-speed, high-resolution scanners for fixed and live cell imaging
Improved depth and photosensitivity with pioneering NIR capabilities and renowned optics
Peace of mind with the reliable, repeatable SilVIR detector
Industry leading ^* ten laser lines with a broader spectral range from 405 nm to 785 nm

*As of October 2023.

Acquire accurate, quantitative image data from the macro scale to subcellular structures
Obtain more information from a single multicolor image
Monitor neuron and other essential dynamics with high-speed imaging